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D9780

Lambda DNA Hind III Digest

for DNA electrophoresis

Synonym(s):

Hind III digested bacteria phage Lambda DNA

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Size/SKUAvailabilityPrice
20 μg
Please contact Customer Service for Availability
$50.50
0.1 mg
Please contact Customer Service for Availability
$137.00
0.5 mg
Please contact Customer Service for Availability
$537.00
1 mg
Please contact Customer Service for Availability
$1,030.00

About This Item

NACRES:
NA.25
UNSPSC Code:
41141827
Form:
liquid
Storage temp.:
−20°C

$50.50


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grade

Molecular Biology

Quality Segment

form

liquid

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

shipped in

dry ice

storage temp.

−20°C

General description

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Other Notes

Contains 8 fragments, 125–23,130 bp
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

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This Item
D9281D2916D0672
grade

Molecular Biology

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

form

liquid

form

liquid

form

buffered aqueous solution

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

usage

100 uses (0.1 mg size), 20 uses (20 μg size), 1000 uses (1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 20 uses (20 μg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)


Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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