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F3520

Anti-von Willebrand Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Von Willebrand Factor Antibody, Von Willebrand Factor Antibody - Anti-von Willebrand Factor antibody produced in rabbit, Anti-Factor VIII-Related Antigen

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À vous/RéférenceDisponibilitéPrix
25 μL
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152,00 €
100 μL
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519,00 €
441,15 €
200 μL
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635,00 €

A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC (p)
Citations:
100

152,00 €


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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling), indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VWF(7450)

General description

Anti-von Willebrand Factor is produced in rabbit using purified von Willebrand factor (factor VIII R:Ag) as the immunogen.
Human von Willebrand factor (factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Immunogen

von Willebrand Factor (Factor VIII-related antigen) from human plasma.

Application

Anti-von Willebrand Factor antibody produced in rabbit has been used for:
  • western blot analysis and immunostaining
  • immunocytochemistry
  • immunohistochemistry
  • indirect immunofluorescence

Biochem/physiol Actions

It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin′s or ethanol-fixed and paraffin-embedded tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Whole antiserum is purified to provide an IgG fraction of antiserum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cet article
HPA001815F7799A2052
Quality Level

300

Quality Level

100

Quality Level

200

Quality Level

200

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

rat, Drosophila, wide range

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling), indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue

technique(s)

immunohistochemistry: 1:50- 1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colorectal carcinoma tissue, western blot: 1:1,000 using nuclear extract of a phorbol ester-treated human A431 cells or nuclear extract of human A431 cells

technique(s)

dot blot: 1:10,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum


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