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Anti-von Willebrand Factor antibody produced in rabbit

Name: Anti-von Willebrand Factor antibody produced in rabbit
Brand: SIGMA
Price: 248 CAD

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Von Willebrand Factor Antibody, Von Willebrand Factor Antibody - Anti-von Willebrand Factor antibody produced in rabbit, Anti-Factor VIII-Related Antigen

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Size/SKU	Availability	Price
25 μL	Please contact Customer Service for Availability	$248.00
100 μL	Please contact Customer Service for Availability	$608.00 $516.80
200 μL	Please contact Customer Service for Availability	$819.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162556

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, IHC (p)

Citations:

100

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Properties

biological source

rabbit

Quality Segment

300

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling), indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue

UniProt accession no.

P04275

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VWF(7450)

Description

General description

Anti-von Willebrand Factor is produced in rabbit using purified von Willebrand factor (factor VIII R:Ag) as the immunogen.

Human von Willebrand factor (factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Immunogen

von Willebrand Factor (Factor VIII-related antigen) from human plasma.

Application

Anti-von Willebrand Factor antibody produced in rabbit has been used for:

western blot analysis and immunostaining
immunocytochemistry
immunohistochemistry
indirect immunofluorescence

Biochem/physiol Actions

It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.

The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin′s or ethanol-fixed and paraffin-embedded tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Whole antiserum is purified to provide an IgG fraction of antiserum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	HPA002082	F3648	F7799
$Anti-von Willebrand Factor antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F3520 Anti-von Willebrand Factor antibody produced in rabbit	Sigma-Aldrich HPA002082 Anti-VWF antibody produced in rabbit	Sigma-Aldrich F3648 Anti-Fibronectin antibody produced in rabbit	$Anti-c-Fos antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich F7799 Anti-c-Fos antibody produced in rabbit
Quality Level 300	Quality Level 100	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling), indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue	technique(s) immunohistochemistry: 1:1000-1:2500, western blot: 0.04-0.4 μg/mL	technique(s) indirect ELISA: 1:10,000 using human fibronectin, indirect immunofluorescence: 1:400 using human foreskin cultured fibroblasts, microarray: suitable, western blot: 1:1,000 using human plasma fibronectin	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colorectal carcinoma tissue, western blot: 1:1,000 using nuclear extract of a phorbol ester-treated human A431 cells or nuclear extract of human A431 cells
Gene Information human ... VWF(7450)	Gene Information human ... VWF(7450)	Gene Information human ... FN1(2335)	Gene Information human ... FOS(2353)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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