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Anti-Glutamine Synthetase antibody produced in rabbit

Name: Anti-Glutamine Synthetase antibody produced in rabbit
Brand: SIGMA
Price: 208 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Glutamine Synthetase Antibody, Glutamine Synthetase Antibody - Anti-Glutamine Synthetase antibody produced in rabbit

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$208.00
100 μL	Available to ship TODAYfromMILWAUKEE	$602.00
0.2 mL	Available to ship TODAYfromMILWAUKEE	$753.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01322097

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IHC (p), WB

Citations:

100

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 45 kDa

species reactivity

rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections, microarray: suitable, western blot: 1:10,000 using rat brain cytosolic fraction

UniProt accession no.

P09606

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GLUL(2752)
mouse ... Glul(14645)
rat ... Glul(24957)

Description

General description

Anti-Glutamine Synthetase is produced in rabbit using a synthetic peptide corresponding to the C-terminus of mouse glutamine synthetase (GS) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. This sequence is identical in human, bovine, rat, hamster and pig GS, and highly conserved in chicken GS. GS is found in mammals as an octamer of identical 45 kDa subunits. It is localized in glial cells of various species. It is present in the brain primarily in astrocytes and in the retina in Muller glial cells that support neurons using glutamate as a neurotransmitter. GS is found in several brain regions including hippocampus, cerebral cortex, neostriatum and cerebellar granular layer, corresponding to sites with high glutamate receptor density.

Immunogen

synthetic peptide corresponding to the C-terminal region of mouse glutamine synthetase (GS) (amino acids 357-373. This sequence is identical in human, bovine, rat, hamster, and pig GS and highly conserved chicken
Gs (single amino acid substitution).

Application

Immunoblotting: a minimum working antibody dilution of 1:10,000 is determined using a rat brain cytosolic fraction extract.
Immunohistochemistry: a minimum working antibody dilution of 1:10,000 is determined using formalin-fixed, paraffin-embedded sections of rat brain.

Anti-Glutamine Synthetase antibody has been used for the detection and localization of GS by immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Anti-Glutamine synthetase recognizes rat glutamine synthetase (GS) (45 kDa). Staining of GS in immunoblotting is specifically inhibited with the GS immunizing peptide (amino acids 357-373 with Nterminally added lysine).

Glutamine synthetase (GS) catalyzes the amidation of glutamate to form glutamine. In the brain, GS is considered as a key enzyme, participating in recycling of the excitatory neurotransmitter glutamate released from neurons and termination of the neurotransmitter signal, as well as in ammonia detoxification. GS activity is a useful marker for astrocytes and an important differentiation feature in the retina. Upregulation of GS is a hallmark of reactive astrocytosis and GS activity level increases following spinal cord injury. The GS expression in astrocytes is diminished in brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. GS is upregulated in a subset of human hepatocellular carcinomas, suggesting that its expression may be related to tumor recurrence, since tumors expressing GS are independent of the extracellular glutamine supply.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MABN1182	MAB302	HPA007316
$Anti-Glutamine Synthetase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G2781 Anti-Glutamine Synthetase antibody produced in rabbit	Sigma-Aldrich MABN1182 Anti-Glutamine Synthetase Antibody, clone 11A3.1	Sigma-Aldrich MAB302 Anti-Glutamine Synthetase Antibody, clone GS-6	Sigma-Aldrich HPA007316 Anti-GLUL antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description clone 11A3.1, from mouse	description clone GS-6, Chemicon^®, from mouse	description Ab1, Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source mouse	biological source mouse	biological source rabbit
antibody form IgG fraction of antiserum	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
species reactivity rat	species reactivity human, rat	species reactivity human, rat, mouse, sheep	species reactivity human
UniProt accession no. P09606	UniProt accession no. P09606	UniProt accession no. P15104	UniProt accession no. P15104

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

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