Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

G2781

Anti-Glutamine Synthetase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Glutamine Synthetase Antibody, Glutamine Synthetase Antibody - Anti-Glutamine Synthetase antibody produced in rabbit

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
25 μL

Available to ship TODAYfromMILWAUKEE

$201.00
100 μL

Available to ship TODAYfromMILWAUKEE

$581.00
0.2 mL

Available to ship TODAYfromMILWAUKEE

$727.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01322097
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
100

$201.00

Available to ship TODAYDetails

​
Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 45 kDa

species reactivity

rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections, microarray: suitable, western blot: 1:10,000 using rat brain cytosolic fraction

UniProt accession no.

P09606

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GLUL(2752)
mouse ... Glul(14645)
rat ... Glul(24957)

General description

Anti-Glutamine Synthetase is produced in rabbit using a synthetic peptide corresponding to the C-terminus of mouse glutamine synthetase (GS) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. This sequence is identical in human, bovine, rat, hamster and pig GS, and highly conserved in chicken GS. GS is found in mammals as an octamer of identical 45 kDa subunits. It is localized in glial cells of various species. It is present in the brain primarily in astrocytes and in the retina in Muller glial cells that support neurons using glutamate as a neurotransmitter. GS is found in several brain regions including hippocampus, cerebral cortex, neostriatum and cerebellar granular layer, corresponding to sites with high glutamate receptor density.

Immunogen

synthetic peptide corresponding to the C-terminal region of mouse glutamine synthetase (GS) (amino acids 357-373. This sequence is identical in human, bovine, rat, hamster, and pig GS and highly conserved chicken
Gs (single amino acid substitution).

Application


Immunoblotting: a minimum working antibody dilution of 1:10,000 is determined using a rat brain cytosolic fraction extract.
Immunohistochemistry: a minimum working antibody dilution of 1:10,000 is determined using formalin-fixed, paraffin-embedded sections of rat brain.
Anti-Glutamine Synthetase antibody has been used for the detection and localization of GS by immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Anti-Glutamine synthetase recognizes rat glutamine synthetase (GS) (45 kDa). Staining of GS in immunoblotting is specifically inhibited with the GS immunizing peptide (amino acids 357-373 with Nterminally added lysine).
Glutamine synthetase (GS) catalyzes the amidation of glutamate to form glutamine. In the brain, GS is considered as a key enzyme, participating in recycling of the excitatory neurotransmitter glutamate released from neurons and termination of the neurotransmitter signal, as well as in ammonia detoxification. GS activity is a useful marker for astrocytes and an important differentiation feature in the retina. Upregulation of GS is a hallmark of reactive astrocytosis and GS activity level increases following spinal cord injury. The GS expression in astrocytes is diminished in brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. GS is upregulated in a subset of human hepatocellular carcinomas, suggesting that its expression may be related to tumor recurrence, since tumors expressing GS are independent of the extracellular glutamine supply.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
G9269G5163HPA007571
Anti-Glutamine Synthetase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

G2781

Anti-Glutamine Synthetase antibody produced in rabbit

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

G9269

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

G5163

Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit

Anti-GLUL antibody produced in rabbit Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

HPA007571

Anti-GLUL antibody produced in rabbit

description

IgG fraction of antiserum, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Gene Information

human ... GLUL(2752)
mouse ... Glul(14645)
rat ... Glul(24957)

Gene Information

human ... GFAP(2670)
rat ... Gfap(24387)

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Gene Information

human ... GLUL(2752)

mol wt

antigen 45 kDa

mol wt

-

mol wt

antigen 65-67 kDa

mol wt

-

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters