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Anti-GRP94 (C-terminal) antibody produced in rabbit

Name: Anti-GRP94 (C-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 653 USD
Availability: InStock

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Endoplasmin precursor, Anti-Glucose Regulated Protein 94, Anti-Tumor Rejection Antigen 1, Anti-gp96

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$653.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01322081

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, ICC, IF, IP, WB

Citations:

$653.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 94 kDa

species reactivity

canine, human, mouse

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): 10-20 μg using MDCK whole cell lysate, indirect immunofluorescence: 2-4 μg/mL using HeLa cells, microarray: suitable, western blot: 0.5-1 μg/mL using whole cell extracts of HeLa, MDCK, and NIH3T3 cells

UniProt accession no.

P14625

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSP90B1(7184)
mouse ... Hsp90b1(22027)
rat ... Tra1(298957)

Description

Immunogen

synthetic peptide corresponding to amino acids 733-750 located near the C-terminus of human GRP94. This sequence is identical in mouse, rat, dog, porcine, and bovine GRP94 and highly conserved (1 amino acid substitutuion) in chicken.

Application

Anti-GRP94 (C-terminal) antibody produced in rabbit has been used in immunocytochemistry.

Anti-GRP94 (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using whole cell extracts of the human epitheloid carcinoma HeLa cells, Madin Darby canine kidney (MDCK) cells, and mouse fibroblast NIH3T3 cells. It is also suitable for immunoprecipitation of GRP94 protein from a MDCK whole cell lysate at 10-20μg concentration.

Biochem/physiol Actions

GRP94 (glucose-regulated protein 94) is involved in protein folding, sorting and secretion, and binding of immunogenic peptides. GRP94 has a role in peptide antigen presentation to major histocompatibility complex class I molecules of antigen presenting cells (APCs). It binds specifically to CD91 (α2-macroglobulin receptor), and possibly to the Toll-like receptors (TLRs) on the surface of APCs. Overexpression of GRP94 has been associated with cellular transformation and tumorigenesis.

GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing, depletion of Ca²⁺ stores, acidosis, and hypoxia conditions that are also common in poorly vascularized tumor tissues.

The GRP94 (glucose-regulated protein 94) is a 94 kDa Ca²⁺- binding glycoprotein that belongs to a subfamily of the heat shock proteins Hsp90. It is a chaperone protein constitutively localized to the endoplasmic reticulum (ER) of mammalian cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G8918	G4048	G7295
Sigma-Aldrich G4420 Anti-GRP94 (C-terminal) antibody produced in rabbit	$Anti-GRP78/BiP (GL-19) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G8918 Anti-GRP78/BiP (GL-19) antibody produced in rabbit	Sigma-Aldrich G4048 Anti-GLUT4 (C-terminal) antibody produced in rabbit	Sigma-Aldrich G7295 Anti-GM130 (C-terminal) antibody produced in rabbit
description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Gene Information human ... HSP90B1(7184) mouse ... Hsp90b1(22027) rat ... Tra1(298957)	Gene Information human ... HSPA5(3309) mouse ... Hspa5(14828)	Gene Information human ... SLC2A4(6517) mouse ... Slc2a4(20528) rat ... Slc2a4(25139)	Gene Information human ... GOLGA2(2801) mouse ... Golga2(99412) rat ... Golga2(64528)
technique(s) western blot: 0.5-1 μg/mL using whole cell extracts of HeLa, MDCK, and NIH3T3 cells, immunocytochemistry: suitable, indirect immunofluorescence: 2-4 μg/mL using HeLa cells, immunoprecipitation (IP): 10-20 μg using MDCK whole cell lysate, microarray: suitable	technique(s) immunocytochemistry: 1:1,000 using mouse fibroblasts NIH3T3 cells, western blot: 1:3,000 using human epitheloid carcinoma HeLa whole cell extract, microarray: suitable	technique(s) western blot: 1-2 μg/mL using C2C12 cell lysate and HepG2 cell lysate, indirect immunofluorescence: 15-20 μg/mL using C2C12 cells	technique(s) western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of rat NRK cells, immunoprecipitation (IP): 2-4 μg using Hela and 3T3 cell extracts., indirect immunofluorescence: 0.2-0.4 μg/mL using rat NRK cells

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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