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Anti-Growth Factor Independence-1 antibody, Mouse monoclonal

Name: Anti-Growth Factor Independence-1 antibody, Mouse monoclonal
Brand: SIGMA
Price: 842.35 CAD

clone 2.5D.17, purified from hybridoma cell culture

Synonym(s):

Anti-GFI1, Anti-ZNF163

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Size/SKU	Availability	Price
200 μL	Please contact Customer Service for Availability	$991.00 $842.35

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02263329

Conjugate:

unconjugated

Clone:

2.5D.17, monoclonal

Application:

ARR, IHC, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2.5D.17, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, mouse, human

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable, microarray: suitable, western blot: 1-2 μg/mL using total cell extract of A549 cells

isotype

IgG1

UniProt accession no.

Q99684

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)

Description

General description

Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence

Immunogen

rat growth factor independence-1 (GFI1).

Application

Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:

immunoblotting
immunohistochemistry
EMSA (electrophoretic mobility shift analysis)

Biochem/physiol Actions

Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.

The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

The antibody may also be used in EMSA (electrophoretic mobility shift analysis).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M7815	C7855	H4538
Sigma-Aldrich G6670 Anti-Growth Factor Independence-1 antibody, Mouse monoclonal	Sigma-Aldrich M7815 Anti-MDM2 antibody ,Mouse monoclonal	Sigma-Aldrich C7855 Anti-Cdh1 antibody,Mouse monoclonal	Sigma-Aldrich H4538 Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal
description clone 2.5D.17, purified from hybridoma cell culture	description clone HDM2-323, purified from hybridoma cell culture	description clone DCS-266, purified from hybridoma cell culture	description clone HDAC5-35, purified from hybridoma cell culture
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... GFI1(2672) mouse ... Gfi1(14581) rat ... Gfi1(24388)	Gene Information human ... MDM2(4193) mouse ... Mdm2(17246)	Gene Information human ... CDH1(999)	Gene Information human ... HDAC5(10014) mouse ... Hdac5(15184) rat ... Hdac5(84580)
clone 2.5D.17, monoclonal	clone HDM2-323, monoclonal	clone DCS-266, monoclonal	clone HDAC5-35, monoclonal
species reactivity rat, mouse, human	species reactivity human, mouse	species reactivity human	species reactivity rat, mouse, human
biological source mouse	biological source mouse	biological source mouse	biological source mouse

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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