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Anti-GRP78/BiP (ET-21) antibody produced in rabbit

Name: Anti-GRP78/BiP (ET-21) antibody produced in rabbit
Brand: SIGMA
Price: 635 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Enhanced Validation

Synonym(s):

Anti-78-kDa Glucose-Regulating Protein, Anti-Immunoglobulin Heavy Chain Binding Protein

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$635.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01865530

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, IHC, WB

Citations:

$635.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 78 kDa

species reactivity

mouse, chicken, Xenopus, hamster, human, rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: 1:1,000 using methanol/acetone fixed cultured mouse fibroblast NIH3T3 cell line, immunohistochemistry: 1:1,000 using human cerebral cortex and human thyroid gland tissue sections, western blot: 1:3,000 using whole cell extract of human epitheloid carcinoma HeLa cell line

UniProt accession no.

P11021

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPA5(3309)
mouse ... Hspa5(14828)
rat ... Hspa5(25617)

Description

Application

Anti-GRP78/BiP (ET-21) antibody produced in rabbit is suitable for use as a primary antibody:

for immunofluorescence staining of frozen heart tissue to examine whether ER stress signaling activation occurs in cardiomyocytes
for immunohistochemistry on Paraffin embedded sections of human cerebral cortex and human thyroid gland tissue sections.
to detect Bip by immunoblotting of protein extract from WT AB strain of zebrafish (Danio rerio)
at a working dilution of 1:1000 to detect GRP78 in extract of prostate cancer cells after androgen deprivation therapy

It is also suitable for immunoblotting at a working dilution of 1:3000 using human epitheloid carcinoma HeLa whole cell extract and for immunocytochemistry at a working dilution of 1:1000 using mouse fibroblasts NIH3T3 cells.

Biochem/physiol Actions

GRP78/BiP levels are elevated in Alzheimer′s disease brains and the decreased expression of GRP78/BiP is found associated with missense mutations in presenilin-1 (PS-1).

The GRP78 (78-kDa glucose-regulated protein) is a member of the heat shock proteins Hsp70 required for cell viability. It is a Ca²⁺-binding molecular chaperone that is localized to the endoplasmic reticulum (ER). It plays a key role in proper glycosylation, folding and assembly of newly synthesized membrane bound or secretory proteins. It also facilitates retention of mutant or defective proteins that are improperly folded and prevents translocation of such proteins from the cytosol to the ER lumen. The protein is induced under conditions of stress such as oxidative stress, chemical toxicity, glycosylation and hypoxia. It plays a crucial role in the maintenance of cell homeostasis and the prevention of apoptosis and acts as a biomarker of hypoglycemia. It has a neuroprotective function in neurons exposed to glutamate exotoxicity and oxidative stress.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G8918	SAB5700613	SAB4501452
$Anti-GRP78/BiP (ET-21) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G9043 Anti-GRP78/BiP (ET-21) antibody produced in rabbit	$Anti-GRP78/BiP (GL-19) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G8918 Anti-GRP78/BiP (GL-19) antibody produced in rabbit	Sigma-Aldrich SAB5700613 Anti-GRP78/BiP antibody produced in rabbit	Sigma-Aldrich SAB4501452 Anti-GRP78 antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description -	description affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
mol wt antigen 78 kDa	mol wt antigen 78 kDa	mol wt 72	mol wt antigen 72 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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