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Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse

Name: Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse
Brand: SIGMA
Price: 803 USD
Availability: InStock

clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate Dehydrogenase

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$803.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD09265360

Conjugate:

peroxidase conjugate

Clone:

GAPDH-71.1, monoclonal

Application:

Citations:

234

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

GAPDH-71.1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~37 kDa

species reactivity

rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken

should not react with

prokaryotes

technique(s)

western blot: 1:35,000-1:70,000 using A431 total cell extract

isotype

IgM

UniProt accession no.

P04406

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)

Description

General description

Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH.

The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.

Immunogen

rabbit GAPDH.

Application

Monoclonal Anti-GAPDH-Peroxidase antibody produced in mouse has been used in

immunoblotting
enzyme linked immunosorbent assay (ELISA)
immunocytochemistry

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.

The antibody does not crossreact with non-vertebrate and prokaryote GAPDH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	G8795	G9545	ABS16
Sigma-Aldrich G9295 Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse	Sigma-Aldrich G8795 Anti-GAPDH antibody, Mouse monoclonal	Sigma-Aldrich G9545 Anti-GAPDH antibody produced in rabbit	Sigma-Aldrich ABS16 Anti-GAPDH Antibody
description clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution	description clone GAPDH-71.1, purified from hybridoma cell culture	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description from rabbit, purified by affinity chromatography
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture, purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate peroxidase conjugate	conjugate unconjugated	conjugate unconjugated	conjugate -
species reactivity rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken	species reactivity bovine, turkey, canine, chicken, monkey, mink, mouse, human, rabbit, rat, hamster	species reactivity mouse, rat, human	species reactivity mouse, human, rat
clone GAPDH-71.1, monoclonal	clone GAPDH-71.1, monoclonal	clone polyclonal	clone polyclonal
Gene Information human ... GAPDH(2597) mouse ... Gapdh(14433) rat ... Gapdh(24383)	Gene Information human ... GAPDH(2597) mouse ... Gapdh(14433) rat ... Gapdh(24383)	Gene Information human ... GAPDH(2597) mouse ... Gapdh(14433) rat ... Gapdh(24383)	Gene Information human ... GAPDH(2597) mouse ... Gapdh(14433)
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Precautionary Statements

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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