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Anti-Myosin Iβ (Nuclear) antibody produced in rabbit

Name: Anti-Myosin Iβ (Nuclear) antibody produced in rabbit
Brand: SIGMA
Price: 905 CAD

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Myh 2, Anti-Myo1c, Anti-Myr 2

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Size/SKU	Availability	Price
200 μL	Please contact Customer Service for Availability	$905.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD05665241

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

$905.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~120 kDa

species reactivity

human, mouse, canine, rat

technique(s)

indirect immunofluorescence: 1:50 using mouse NIH3T3 cells, microarray: suitable, western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells

UniProt accession no.

O00159

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYO1C(4641)
mouse ... Myo1c(17913)
rat ... Myo1c(65261)

Description

General description

Myosins belong to a superfamily of actin-based motor proteins comprising to date at least 15 classes. Myosin I (also designated myosin-1c, myo1c, M1C, myr 2 and myh 2) is a widely distributed single-headed myosin composed of an N-terminal motor domain, a calmodulin-light chain binding neck region, and a short C-terminal domain. In mammalian cells, myosin I is usually found in the cytoplasm and is especially enriched in perinuclear regions and dynamic cell margins. Myosin Iβ is found in a variety of cells such as kidney tubular cells and inner-ear hair cells.

Immunogen

synthetic peptide corresponding to amino acid residues 1-16 of the heavy chain of mouse myosin Iβ (nuclear form). This sequence is missing from the other myosin I isoforms as well as from other currently known myosins.

Application

Anti-Myosin Iβ (Nuclear) antibody produced in rabbit has been used in:

western blot
confocal immunofluorescence
immunogold labeling
immunofluorescence
co-immunoprecipitation experiments
immunostaining

Biochem/physiol Actions

Myosin I is involved in cell motility, microvilli anchorage, vesicular and organelle transport and signal transduction. Myosin I associate with lipid membranes through its tail domain while its motor domain interacts with actin filaments. Myosin Iβ (nuclear) co-localizes with RNA polymerases I and II and possibly acts as a molecular motor to power transcription.

Recognizes the heavy chain of the nuclear form of human myosin Iβ. Additional bands may be detected in some preparations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M0691	M8064	M4812
Sigma-Aldrich M3567 Anti-Myosin Iβ (Nuclear) antibody produced in rabbit	Sigma-Aldrich M0691 Anti-Myosin VI antibody, Mouse monoclonal	Sigma-Aldrich M8064 Anti-Myosin IIA, non muscle antibody produced in rabbit	Sigma-Aldrich M4812 Anti-Myosin Va (LF-18) antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description ~1 mg/mL, clone MUD-19, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description ~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) indirect immunofluorescence: 1:50 using mouse NIH3T3 cells, western blot: 1:1,000 using extract of human HeLa cells nuclei and dog MDCK cells, microarray: suitable	technique(s) immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 2 μg/mL using A431 total cell extract	technique(s) indirect immunofluorescence: 1:100 using cultured rat NRK cells, microarray: suitable, western blot: 1:1,000 using whole cell extracts of cultured dog MDCK kidney cells and cultured human Jurkat cells	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections, microarray: suitable, western blot: 1:1,000 using a rat brain extract
mol wt antigen ~120 kDa	mol wt antigen ~150 kDa by SDS-PAGE	mol wt antigen ~200 kDa	mol wt antigen 190 kDa

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c