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M5755

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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Size/SKUAvailabilityPrice
1 L

Available to ship TODAYfromMILWAUKEE

$315.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$315.00

Available to ship TODAYDetails

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grade

Molecular Biology

Quality Segment

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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This Item
T8280PPB00893290
MOPS-EDTA-Sodium Acetate (MESA) powder for RNA electrophoresis buffers

Sigma-Aldrich

M5755

MOPS-EDTA-Sodium Acetate (MESA)

Tris Acetate-EDTA buffer 10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T8280

Tris Acetate-EDTA buffer

Tris Acetate-EDTA buffer pH 8.3, pHast Pack™, powder

Sigma-Aldrich

PPB008

Tris Acetate-EDTA buffer

TRIS borate – EDTA buffer solution for Northern and Southern blotting, solution

Sigma-Aldrich

93290

TRIS borate – EDTA buffer solution

description

powder for RNA electrophoresis buffers

description

10× concentrate, BioReagent, Molecular Biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

description

pH 8.3, pHast Pack™, powder

description

for Northern and Southern blotting, solution

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

Molecular Biology

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

pHast Pack™

product line

BioReagent

form

powder

form

powder blend

form

powder

form

solution

solubility

H2O: soluble, faintly yellow

solubility

-

solubility

-

solubility

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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