M7060
Monoclonal Anti-Moesin antibody produced in mouse
clone 38/87, purified immunoglobulin, buffered aqueous solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 mL | Please contact Customer Service for Availability | $829.00 |
About This Item
Conjugate:
unconjugated
Clone:
38/87, monoclonal
Application:
ELISA (i), FACS, ICC, IHC (p), IP, WB
Citations:
4
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
38/87, monoclonal
form
buffered aqueous solution
mol wt
antigen 78-80 kDa
species reactivity
human, bovine, rat, mouse, pig
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.5-1 μg/mL using whole extract of cultured human acute T cell leukemia Jurkat cells
isotype
IgG1
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MSN(4478)
mouse ... Msn(17698)
rat ... Msn(81521)
Immunogen
bovine uterus moesin.
Application
Mouse monoclonal clone 38/87 anti-Moesin antibody may be used for ELISA, immunoblotting (a closely spaced doublet of 78/80 kDa) immunoprecipitation, immunohistochemistry (reported for frozen sections and for formalin-fixed paraffin-embedded sections following boiling in 0.01M citrate buffer pH 6), immunocytochemistry (3.7% paraformaldehyde, 0.1% Triton X-100), flow cytometry (3-3.7% paraformaldehyde, 0.1-0.25% Triton X-100) and electron microscopy. The intensity of the fainter doublet‘s upper band seen in immunoblotting varies depending on the tissue. The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.
Biochem/physiol Actions
Mouse monoclonal clone 38/87 anti-Moesin antibody recognizes the 78 kDa moesin and 80 kDa radixin molecules. It does not react with talin or ezrin. Reactivity has been reported with human, bovine, porcine, rat and mouse moesin.
The ERM (ezrin-radixin-moesin) proteins, members of the talin-protein 4.1- merlin/schwannomin superfamily, are general cross-linkers between the plasma membrane and actin filaments. They provide such links through their N-terminal halves that associate with integral membrane proteins either directly or indirectly through adapter molecules, and through their C-terminal halves that associate with F-actin. ERM proteins are involved not only in cytoskeletal organization but also in signal transduction and apoptosis. Because their expression is regulated in a tissue-specific manner, each ERM protein has been proposed to have unique functions. There is approximately 80% homology between moesin, ezrin and radixin. These proteins are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures. However, moesin (in contrast to radixin and ezrin) is strongly expressed in endothelium of vessels, in T and B lymphocytes, in basal layers of squamous epithelium and glandular ducts, and in cells of carcinoma and mesothelioma. Moesin is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines. It is overexpressed in some estrogen receptor (ER)-negative cell lines but absent from ER-positive ones, suggesting that it may play a role in the invasiveness and pattern of metastasis characteristic of ER-negative breast cancers.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form purified from hybridoma cell culture |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| clone 38/87, monoclonal | clone Men-8, monoclonal | clone GS-2C4, monoclonal | clone GS-2C4, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Gene Information human ... MSN(4478) | Gene Information human ... MECP2(4204) | Gene Information human ... GSN(2934) | Gene Information human ... GSN(2934) |
| species reactivity human, bovine, rat, mouse, pig | species reactivity mouse, human, rat, monkey | species reactivity bovine, pig, human, rabbit | species reactivity rabbit, canine, human, mouse, monkey |
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Storage Class
10 - Combustible liquids
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Related Content
Datasheet
Mei Wang et al.
International journal of biological sciences, 18(3), 942-955 (2022-02-18)
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Cancer multidrug resistance (MDR) occurs when cancer cells evade the cytotoxic actions of chemotherapeutics through the active efflux of drugs from within the cells. Our group have previously demonstrated that multidrug-resistant breast cancer cells spontaneously shed microparticles (MPs) and that
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M7060-.2ML | 04061837720826 |