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Anti-MBD2a,b (RA-18) antibody produced in rabbit

Name: Anti-MBD2a,b (RA-18) antibody produced in rabbit
Brand: SIGMA
Price: 858 CAD

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-DMTase, Anti-NY-CO-41

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Size/SKU	Availability	Price
200 μL	Please contact Customer Service for Availability	$858.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD06798469

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

$858.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells, microarray: suitable, western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells

UniProt accession no.

Q9UBB5

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MBD2(8932)
mouse ... Mbd2(17191)

Description

General description

Anti-MBD2a,b (RA-18) is developed in rabbit using as immunogen a synthetic peptide corresponding human MBD2a conjugated to KLH. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene; MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a is a component of the MeCP1 corepressor complex, which is a 400-800 kDa complex containing as components MBD2, Mi-2, MTA2, MBD3, and HDAC1/2.

The gene encoding methyl-CpG binding domain protein 2 (MBD2a) is localized on chromosome 18.

Immunogen

synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3.

Application

Anti-MBD2a,b (RA-18) antibody produced in rabbit has been used:

for chromatin immunoprecipitation and western blotting
immunofluorescence
immunoblotting

Biochem/physiol Actions

MBD2 binds to methylated DNA in vitro or in vivo, suggesting that MBD2 targets the MeCP1 complex to methylated DNA. MBD2a interacts with RNA Helicase A (RHA). MBD2 protein appear to protect against some cancers.

Methyl-CpG binding domain protein 2 (MBD2a) acts as a repressor during transcription.

The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200201	G6919	HPA067615
Sigma-Aldrich M7318 Anti-MBD2a,b (RA-18) antibody produced in rabbit	Sigma-Aldrich SAB4200201 Anti-Cyclophilin B antibody produced in rabbit	Sigma-Aldrich G6919 Anti-G9a Methyltransferase antibody produced in rabbit	Sigma-Aldrich HPA067615 Anti-MBD2 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description ~1.0 mg/mL, affinity isolated antibody	description affinity isolated antibody, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
technique(s) indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells, western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells, microarray: suitable	technique(s) indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells, western blot: 0.5-1.0 μg/mL using whole extracts of human HepG2 and rat NRK cells	technique(s) immunoprecipitation (IP): 1-2 μg using 293-T cell extracts, microarray: suitable, western blot: 1-2 μg/mL using nuclear extracts of 293-T cells	technique(s) ChIP: 1—10 μg (per reaction), immunofluorescence: 0.25-2 μg/mL

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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