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M7318

Anti-MBD2a,b (RA-18) antibody produced in rabbit

Name: Anti-MBD2a,b (RA-18) antibody produced in rabbit
Brand: SIGMA
Price: 588 EUR

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Anti-DMTase, Anti-NY-CO-41

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A voi/SKU	Disponibilità	Prezzo
200 μL	Per informazioni sulla disponibilità, contatta il Servizio Clienti.	588,00 €

Informazioni su questo articolo

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD06798469

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, WB

Citations:

588,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

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Proprietà

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells, microarray: suitable, western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells

UniProt accession no.

Q9UBB5

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MBD2(8932)
mouse ... Mbd2(17191)

Descrizione

General description

Anti-MBD2a,b (RA-18) is developed in rabbit using as immunogen a synthetic peptide corresponding human MBD2a conjugated to KLH. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene; MBD2a is a 414 amino acids protein, whereas MBD2b lacks the 152 amino acids N-terminal extension. MBD2a is a component of the MeCP1 corepressor complex, which is a 400-800 kDa complex containing as components MBD2, Mi-2, MTA2, MBD3, and HDAC1/2.

The gene encoding methyl-CpG binding domain protein 2 (MBD2a) is localized on chromosome 18.

Immunogen

synthetic peptide corresponding to amino acids 294-411 of human MBD2a conjugated to KLH via an N-terminal added lysine residue. This sequence is conserved in MBD2a and MBD2b of human origin, and is not found in MBD3.

Application

Anti-MBD2a,b (RA-18) antibody produced in rabbit has been used:

for chromatin immunoprecipitation and western blotting
immunofluorescence
immunoblotting

Biochem/physiol Actions

MBD2 binds to methylated DNA in vitro or in vivo, suggesting that MBD2 targets the MeCP1 complex to methylated DNA. MBD2a interacts with RNA Helicase A (RHA). MBD2 protein appear to protect against some cancers.

Methyl-CpG binding domain protein 2 (MBD2a) acts as a repressor during transcription.

The antibody recognizes MBD2a (~45 kDa) and MBD2b (~29 kDa). In immunoblotting, an unidentified band at ~43 kDa may also be detected.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo	M9317	D4692	H4539
Sigma-Aldrich M7318 Anti-MBD2a,b (RA-18) antibody produced in rabbit	Sigma-Aldrich M9317 Anti-MeCP2 antibody produced in rabbit	Sigma-Aldrich D4692 Anti-DNMT1 antibody produced in rabbit	Sigma-Aldrich H4539 Anti-Histone Deacetylase 11 (HDAC11) antibody produced in rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
technique(s) indirect immunofluorescence: 6-8 μg/mL using NIH3T3 cells, western blot: 0.5-1.0 μg/mL using nuclear extracts of HeLa cells, microarray: suitable	technique(s) microarray: suitable, western blot: 0.5-1 μg/mL using nuclear extracts of the MCF7 breast carcinoma cell line.	technique(s) microarray: suitable, western blot: 0.5-1.0 μg/mL using nuclear extracts of 293T or HeLa cells	technique(s) western blot (chemiluminescent): 0.5-1 μg/mL using extracts of mouse brain, western blot (chemiluminescent): 4-6 μg/mL using nuclear extracts of HeLa cells.

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Classe di stoccaggio

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c