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Anti-Myopodin antibody produced in rabbit

Name: Anti-Myopodin antibody produced in rabbit
Brand: SIGMA
Price: 471 EUR

affinity isolated antibody, buffered aqueous solution

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Size/SKU	Availability	Price
200 μL	Please contact Customer Service for Availability	€471.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD06798472

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (f), WB

Citations:

€471.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 80 kDa

species reactivity

rat

technique(s)

immunohistochemistry (frozen sections): 1-2 μg/mL using rat skeletal muscle and rat kidney, western blot: 1-2 μg/mL using cytosolic fraction of rat skeletal muscle

UniProt accession no.

D4A702

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SYNPO2(171024)
mouse ... Synpo2(118449)
rat ... Synpo2(499702)

Description

General description

Myopodin (80-95 kDa), a novel actin bundling protein, is an additional member of the synaptopodin gene family. Myopodin is expressed in skeletal and cardiac muscles. Myopodin contains one PPXY motif, multiple PXXP motifs, and a nuclear export sequence (NES). In the-disc, myopodin colocalizes with α-actinin. Myopodin, like several actin-bundling proteins, has been shown to shuttle between the nucleus and cytoplasm. It is localized in nucleus in myoblasts.

Immunogen

synthetic peptide encoding amino acids 566-585 located at the mid-region of human myopodin, conjugated to KLH. This sequence is highly conserved (77% sequence identity) in mouse myopodin and is not found in human or rat synaptopodin.

Application

Anti-Myopodin has been used in:

immunohistochemistry
immunostaining
western blot analysis

Biochem/physiol Actions

Myopodin directly binds to actin and contains an actin-binding site in the centre of the protein. Myopodin has actin bundling activity as shown by lantraculin - a sensitive cytosolic actin bundles and nuclear actin loops in transfected cells expressing GFP-myopodin. It binds to stress fibres in a punctuated pattern into the Z-disc during myotube differentiation. Myopodin is frequently down-regulated in invasive stages of some types of cancers, including invasive bladder tumors. Frequent complete or partial deletions of the myopodin gene have been shown to occur in 80% of invasive prostate cancer cases. Expression of myopodin induces suppression of tumor growth both in vivo and in vitro. Myopodin inhibits tumor metastasis by functioning as a tumor suppressor gene.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	S9442	S4813	N5037
Sigma-Aldrich M9818 Anti-Myopodin antibody produced in rabbit	$Anti-Synaptopodin (SE-19) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich S9442 Anti-Synaptopodin (SE-19) antibody produced in rabbit	$Anti-α1-Syntrophin (RA-16) antibody produced in rabbit buffered aqueous solution, IgG fraction of antiserum$ Sigma-Aldrich S4813 Anti-α1-Syntrophin (RA-16) antibody produced in rabbit	Sigma-Aldrich N5037 Anti-Neurabin II (C-terminal) antibody produced in rabbit
antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
technique(s) immunohistochemistry (frozen sections): 1-2 μg/mL using rat skeletal muscle and rat kidney, western blot: 1-2 μg/mL using cytosolic fraction of rat skeletal muscle	technique(s) microarray: suitable, western blot: 1:3,000 using cytosolic S1 fraction of rat brain	technique(s) immunohistochemistry (frozen sections): suitable using 0.5% paraformaldehyde fixed/0.5% Triton X-100 treated rat skeletal muscle, microarray: suitable, western blot: 1:2,000 using rat skeletal muscle homogenate extract (membrane fraction)	technique(s) immunoprecipitation (IP): 10-20 μg using rat brain extract (S1 fraction), indirect immunofluorescence: 10-20 using MDCK cells., microarray: suitable, western blot: 1-2 μg/mL using mouse and rat brain extracts (S1 fraction)
species reactivity rat	species reactivity rat	species reactivity rat	species reactivity rat, mouse, canine
Gene Information human ... SYNPO2(171024) mouse ... Synpo2(118449) rat ... Synpo2(499702)	Gene Information human ... SYNPO(11346) rat ... Synpo(60324)	Gene Information mouse ... Snta1(20648) rat ... Snta1(362242)	Gene Information human ... PPP1R9B(84687) mouse ... Ppp1r9b(217124) rat ... Ppp1r9b(84686)

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c