The immunogen range, 367-383, is located at the C-terminal region of the protein.
P0372
Anti-Podocin antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Podocin Antibody, Podocin Antibody - Anti-Podocin antibody produced in rabbit
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, WB CL
Citations:
145
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~42 kDa (doublet)
species reactivity
human, rat, mouse
technique(s)
indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections, western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NPHS2(7827)
mouse ... Nphs2(170484)
rat ... Nphs2(170672)
General description
Podocin is a podocyte structural protein, that belongs to the band-7-stomatin family of lipid raft-associated proteins. This family contains more than 1800 conserved proteins which share ∼150 amino acid domains showing similarity to mitochondrial protein prohibitin (PHB).
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.
Immunogen
Synthetic peptide corresponding to amino acid residues of human podocin with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.
Application
Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry.
Biochem/physiol Actions
Anti-Podocin recognizes human, mouse, and rat podocin.
Podocin and nephrin are essential for maintaining the slit diaphragm. Studies show that mutations in this gene account for around 15% cases of nephrotic syndrome cases. Podocin interacts with cholesterol and promotes the formation of protein-lipid supercomplexes, which control ion channel complexes. The podocin gene is known to be associated with autosomal recessive SRNS (nephrotic syndrome type 2). Podocin actively participates in the ultrafiltration process occuring in kidney.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level - |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| species reactivity human, rat, mouse | species reactivity rat, human, mouse | species reactivity rat | species reactivity bovine, rat, human, mouse |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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この抗体の42ｋDaのアミノ酸配列についてはどちらに記載ありますでしょうか C末なのか、N末なのか、抗原の場所の確認の仕方を教えてください
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