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Monoclonal Anti-Phosphotyrosine antibody produced in mouse

Name: Monoclonal Anti-Phosphotyrosine antibody produced in mouse
Brand: SIGMA
Price: 691 CAD

clone PT-66, ascites fluid

Synonym(s):

Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr

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Pack Size	SKU	Availability	Price
0.2 mL		Please contact Customer Service for Availability	$691.00

About This Item

NACRES:

NA.44

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

PT-66, monoclonal

Application:

ELISA (i), WB

Citations:

$691.00

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PT-66, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:1,600, western blot: 1:2,000 using a fresh human platelet preparation

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.

Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Phosphorylation, attachment of a phosphate group to protein, alters protein functionality by activation or deactivation of the protein, in which protein kinase plays a crucial role. Tyrosine residue phosphorylation plays a key role in cell growth and differentiation. The monoclonal anti-phosphotyrosine antibody is useful in immunoblotting for identification of phosphotyrosine containing protein from cultured human epidermoid carcinoma cell line A-431 and human platelets preparation. This product can be used in immunofluorescent labeling of tyrosine residue at focal adhesion and cellular junctions of cultured MDCK cells. This product is also useful in studies of signal transduction and growth factor receptors. This antibody has shown specificity for binding to proteins that contain phosphorylated tyrosine residue either in free amino acid form or in conjugation with KLH or BSA but will not react with non-phosphorylated tyrosine or other phosphorylated proteins and amino acids. It will also not react with phosphorylated molecules like ATP or AMP. Monoclonal anti-phosphotyrosine reacts specifically with mouse and human.

Immunogen

phosphotyrosine conjugated to BSA

Application

Monoclonal Anti-Phosphotyrosine antibody produced in mouse has been used in

immunocytochemistry
immunoprecipitation
western blotting
peptide binding studies flow cytometry
enzyme linked immunosorbent assay (ELISA)
radioimmunoassay (RIA)
immunoaffinity isolation

Monoclonal anti-phosphotyrosine antibody produced in mouse is used in immunohistochemisty, flow cytometry, immunoprecipitation, immunoblotting, ELISA and RIA for localization of phosphorylated tyrosine containing proteins. It can also be used for immunoaffinity isolation.

Biochem/physiol Actions

Phosphotyrosine levels enhanced in cellular proteins leads to activation of several cellular processes mediated by phosphotyrosine kinases. Phosphotyrosine residues that are autophosphorylated by the binding of ligands associated with receptors like epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and insulin receptors, functions as the primary integral component of mitogenic signaling cascade. Phosphotyrosine mediates the activation of T-cell as a result of phosphorylation of tyrosine residues by tyrosine kinases in cytoplasmic domains of CD4 an CD8 which in turn phosphorylates TCR-CD3 complex. Phosphotyrosine activity has also been found in many retroviruses whose oncogenes encodes tyrosine-specific protein kinase.

This antibody is specific for phosphorylated tyrosine both as the free amino acid or when conjugated to carriers such as BSA or KLH

Tyrosine residue phosphorylation plays a key role in cell growth and differentiation.

Physical form

This product is supplied as ascites fluid containing 15 mM sodium azide

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	P5872	P1869	A1806
Sigma-Aldrich P3300 Monoclonal Anti-Phosphotyrosine antibody produced in mouse	Sigma-Aldrich P5872 Anti-Phosphotyrosine antibody, Mouse monoclonal	Sigma-Aldrich P1869 Anti-Phosphotyrosine antibody, Mouse monoclonal	Sigma-Aldrich A1806 Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse
antibody form ascites fluid	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
biological source mouse	biological source mouse	biological source -	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate agarose conjugate
clone PT-66, monoclonal	clone PT-66, monoclonal	clone pT-154, monoclonal	clone PT-66, monoclonal
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) indirect ELISA: 1:1,600, western blot: 1:2,000 using a fresh human platelet preparation	technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets	technique(s) direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF	technique(s) immunoprecipitation (IP): suitable

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000476852

0000439635

0000395457

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Global Trade Item Number

SKU	GTIN
P3300-.2ML	04061834367635
P3300-.5ML	04061838164506

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