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Anti-Parkin antibody, Mouse monoclonal

Name: Anti-Parkin antibody, Mouse monoclonal
Brand: SIGMA
Price: 595 USD
Availability: InStock

clone PRK8, purified from hybridoma cell culture

Synonym(s):

Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$595.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02263896

Conjugate:

unconjugated

Clone:

PRK8, monoclonal

Application:

ARR, WB

Citations:

$595.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PRK8, monoclonal

form

buffered aqueous solution

species reactivity

mouse, rat, hamster, human

concentration

~2 mg/mL

technique(s)

microarray: suitable, western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PARK2(5071)
mouse ... Park2(50873)
rat ... Park2(56816)

Description

General description

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.

Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

Immunogen

recombinant human Parkin.

Application

Monoclonal anti-parkin antibody has been used in:

Enzyme linked immunosorbent assay (ELISA)
Immunoblotting
Immunoprecipitation[63}
Immunocytochemistry
to label parkin in pull-down assay

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P5117	AB5112	SAB4200747
Sigma-Aldrich P6248 Anti-Parkin antibody, Mouse monoclonal	Sigma-Aldrich P5117 Anti-Peripherin antibody, Mouse monoclonal	Sigma-Aldrich AB5112 Anti-Parkin Antibody, a.a. 305-323	Sigma-Aldrich SAB4200747 Anti-Neurofilament 200 antibody, Mouse monoclonal
description clone PRK8, purified from hybridoma cell culture	description clone 8G2, purified from hybridoma cell culture	description serum, Chemicon^®	description clone NE14, purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
species reactivity mouse, rat, hamster, human	species reactivity rat, bovine, pig, mouse, rabbit, feline, human	species reactivity rat, human	species reactivity feline, rat, guinea pig, pig, human, mouse, bovine, chicken
clone PRK8, monoclonal	clone 8G2, monoclonal	clone polyclonal	clone NE14, monoclonal
Gene Information human ... PARK2(5071) mouse ... Park2(50873) rat ... Park2(56816)	Gene Information human ... PRPH(5630) mouse ... Prph(19132) rat ... Prph(24688)	Gene Information human ... PARK2(5071)	Gene Information bovine ... Nefh(528842) cat ... Nefh(101087272) chicken ... Nefh(417020) human ... NEFH(4744) mouse ... Nefh(380684) pig ... Nefh(100156492) rat ... Nefh(24587)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form serum	antibody form purified from hybridoma cell culture

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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