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Anti-phospho-PKR (pThr⁴⁵¹) antibody produced in rabbit

Name: Anti-phospho-PKR (pThr<SUP>451</SUP>) antibody produced in rabbit
Brand: SIGMA
Price: 864 USD
Availability: InStock

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-phospho-Interferon-Inducible Double-Stranded RNA-Dependent Protein (pThr⁴⁵¹)

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Size/SKU	Availability	Price
1 vial	Check Cart for Availability	$864.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD03455798

Conjugate:

unconjugated

Clone:

polyclonal

Application:

—

Citations:

$864.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen 65-68 kDa

species reactivity

mouse, human

technique(s)

immunoblotting: suitable using human HeLa cells stimulated with IFNγ and calyculin

UniProt accession no.

P19525

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr451)

Gene Information

human ... EIF2AK2(5610)
mouse ... Eif2ak2(19106)

Description

General description

Protein kinase regulated by RNA (PKR) is an interferon-inducible ser/thr protein kinase, activated by stress signals and double-stranded RNA (dsRNA). In response to viral infection, PKR is activated by dsRNA-mediated dimerization and autophosphorylation. α-subunit of protein synthesis initiation factor 2 (eIF-2α) is well-studied substrate of PKR. Activation by PKR leads to phosphorylation on Ser51 that result in inhibition of translation. Human PKR contains at least 15 autophosphorylation sites. But phosphorylation on threonine 446 and 451 in the PKR activation loop is required ^{in vivo} and in vitro for high-level kinase activity. PKR is implicated in signalling pathways that involve NF-κB, p38, and IRF-3 and is the key effector in IFN-induced anti-viral responses, apoptosis and protein synthesis.
Anti-Phospho-PKR [pThr⁴⁵¹] specifically recognizes PKR phosphorylated on threonine 451 (65-68 kDa). The antibody detects human and mouse PKR [pThr⁴⁵¹].

Immunogen

synthetic phosphopeptide derived from the region of PKR that is phosphorylated on threonine 451.

Application

Anti-phospho-PKR (pThr⁴⁵¹) antibody may be used for detection by immunoblotting at a working dilution of 1:1,000 using human HeLa cells stimulated with IFN·γ and calyculin. For detection by immunoblotting in human HeLa cells, a working concentration of 0.1-1.0 μg/mL is recommended. Detection of phospho-PKR (pThr⁴⁵¹) was done in peritoneal macrophages obtained from thioglycolate-treated mice and human peripheral blood mononuclear cells at a working dilution of 1:500

Physical form

Supplied as a solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide. The amount of the reagent is sufficient for 10 blots.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	S6311	S6436	P0244
Sigma-Aldrich P6493 Anti-phospho-PKR (pThr⁴⁵¹) antibody produced in rabbit	Sigma-Aldrich S6311 Anti-phospho-S6-Kinase (pThr³⁸⁹) antibody produced in rabbit	Sigma-Aldrich S6436 Anti-phospho-S6-Kinase (pThr⁴²¹/pSer⁴²⁴) antibody produced in rabbit	$Anti-PKR (ET-20) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich P0244 Anti-PKR (ET-20) antibody produced in rabbit
description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution	description IgG fraction of antiserum, buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
UniProt accession no. P19525	UniProt accession no. P23443	UniProt accession no. P23443	UniProt accession no. P19525
mol wt antigen 65-68 kDa	mol wt -	mol wt -	mol wt antigen 68 kDa

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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