The volume is approximately 0.276 mL per vial which equates to roughly 1.38 mL total volume for all 5 vials. The number of samples that can be processed is dependent on the sample size. The protocol suggests adding 1 volume of sample to 2-5 volumes of RNA Sample Loading Buffer. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/866/807/r4268dat.pdf
R4268
RNA Sample Loading Buffer
for NA electrophoresis, with ethidium bromide (50 μg/mL)
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| Size/SKU | Availability | Price |
|---|---|---|
5 vials | Check Cart for Availability | $134.00 |
About This Item
$134.00
Check Cart for Availability
grade
Molecular Biology
Quality Segment
form
liquid
concentration
1.25 ×
technique(s)
electrophoresis: suitable, nucleic acid detection: suitable
foreign activity
RNase, none detected
storage temp.
−20°C
General description
RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.
Application
Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.
RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot.
RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot.
RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.
Preparation Note
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.
Other Notes
Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
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This Item | |||
|---|---|---|---|
| description for NA electrophoresis, with ethidium bromide (50 μg/mL) | description for NA electrophoresis, without ethidium bromide | description for NA electrophoresis | description - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade - |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) protein electrophoresis: suitable |
| concentration 1.25 × | concentration - | concentration - | concentration - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| form liquid | form solution | form liquid | form solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. −20°C |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Carc. 1B - Muta. 2 - Repr. 1B - Skin Sens. 1 - STOT RE 2 Oral
Target Organs
Blood
Storage Class
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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