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R5403

Monoclonal Anti-Rhodopsin antibody produced in mouse

clone 1D4, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CSNBAD1, Anti-OPN2, Anti-RP4

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Pack SizeSKUAvailabilityPrice
100 μL
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$697.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01865244
Conjugate:
unconjugated
Clone:
1D4, monoclonal
Application:
ICC, WB
Citations:
18

$697.00

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1D4, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, bovine

technique(s)

immunocytochemistry: 1:1,000 using human retinal samples, western blot: 1:1,000 using Sf9 cells expressing the bovine gene

isotype

IgG1

UniProt accession no.

P08100

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RHO(6010)
rat ... Rho(24717)

General description

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin′s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.

Immunogen

bovine rhodopsin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Mouse monoclonal clone 1D4 anti-Rhodopsin antibody has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation. It is used as a probe to determine the presence and roles of rhodopsin in the conversion of light into electrochemical signals.

Biochem/physiol Actions

By Western blot, mouse monoclonal clone 1D4 anti-Rhodopsin antibody detects an ~40 kDa protein representing rhodopsin from Sf 9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.
The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope, and detects rhodopsin from human and bovine retinal samples.

Physical form

Solution in phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

human, rat, bovine

species reactivity

goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle

species reactivity

rat, human, fish

species reactivity

rat, bovine, chicken, human, mouse

clone

1D4, monoclonal

clone

RET-P1, monoclonal

clone

4D2, monoclonal

clone

G8, monoclonal

Gene Information

human ... RHO(6010)
rat ... Rho(24717)

Gene Information

human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)

Gene Information

human ... RHO(6010)
rat ... Rho(24717)

Gene Information

human ... GRK1(6011)
mouse ... Grk1(24013)
rat ... Grk1(81760)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000535701
0000535701
SLCN3751
SLCN1262
SLCK1383

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Related Content

Data Sheet - R5403

Instructions

Angelo Felline et al.
Computational and structural biotechnology journal, 19, 6020-6038 (2021-12-02)
Failure of a protein to achieve its functional structural state and normal cellular location contributes to the etiology and pathology of heritable human conformational diseases. The autosomal dominant form of retinitis pigmentosa (adRP) is an incurable blindness largely linked to
Jun Yin et al.
Investigative ophthalmology & visual science, 53(8), 4943-4951 (2012-06-30)
In experimental eye research, zebrafish has become a powerful model for human retina disorders. The purpose of the present study is the characterization of antibodies commonly employed in zebrafish models for rod photoreceptor degeneration. The 1D4 monoclonal antibody, developed against
Silvia Ravera et al.
Cells, 12(17) (2023-09-08)
Dysfunction of the retinal pigment epithelium (RPE) is associated with several diseases characterized by retinal degeneration, such as diabetic retinopathy (DR). However, it has recently been proposed that outer retinal neurons also participate in the damage triggering. Therefore, we have
View All Related Papers

Global Trade Item Number

SKUGTIN
R5403-100UL04061837724206

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