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Monoclonal Anti-Rhodopsin antibody produced in mouse

Name: Monoclonal Anti-Rhodopsin antibody produced in mouse
Brand: SIGMA

clone 1D4, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-CSNBAD1, Anti-OPN2, Anti-RP4

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01865244

Conjugate:

unconjugated

Clone:

1D4, monoclonal

Application:

ICC, WB

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1D4, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, bovine

technique(s)

immunocytochemistry: 1:1,000 using human retinal samples, western blot: 1:1,000 using Sf9 cells expressing the bovine gene

isotype

IgG1

UniProt accession no.

P08100

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RHO(6010)
rat ... Rho(24717)

Description

General description

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin′s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.

Immunogen

bovine rhodopsin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Mouse monoclonal clone 1D4 anti-Rhodopsin antibody has been successfully used in Western blot, immunocytochemistry, and immunoprecipitation. It is used as a probe to determine the presence and roles of rhodopsin in the conversion of light into electrochemical signals.

Biochem/physiol Actions

By Western blot, mouse monoclonal clone 1D4 anti-Rhodopsin antibody detects an ~40 kDa protein representing rhodopsin from Sf 9 cells expressing the bovine gene. Immunocytochemical staining of rhodopsin in human retinal samples results in staining of both rod and cone outer segments.

The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope, and detects rhodopsin from human and bovine retinal samples.

Physical form

Solution in phosphate buffered saline containing 1 mg/mL BSA and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	MABN15	R9153	SAB4502636
Sigma-Aldrich R5403 Monoclonal Anti-Rhodopsin antibody produced in mouse	Sigma-Aldrich MABN15 Anti-Rhodopsin Antibody, clone 4D2	Sigma-Aldrich R9153 Anti-Rhodopsin antibody produced in rabbit	Sigma-Aldrich SAB4502636 Anti-Rhodopsin antibody produced in rabbit
species reactivity human, rat, bovine	species reactivity rat, human, fish	species reactivity human	species reactivity mouse, human, rat
Gene Information human ... RHO(6010) rat ... Rho(24717)	Gene Information human ... RHO(6010) rat ... Rho(24717)	Gene Information human ... RHO(6010) mouse ... Rho(212541) rat ... Rho(24717)	Gene Information human ... RHO(6010)
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone 1D4, monoclonal	clone 4D2, monoclonal	clone polyclonal	clone polyclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c