This product has not been validated for use on rat tissue. However, this product recognizes Chondroitin Sulfate-containing Proteoglycans (CSPG) in bovine, human, chicken, and mouse and may also recognize samples from rat. Clone CS-56, has been used with rat tissue in the following citation: Journal of Biomedical Optics, Vol. 16, Issue 2, 027003 (February 2011). https://doi.org/10.1117/1.3549700.
SAB4200696
Anti-Chondroitin Sulfate antibody, Mouse monoclonal
clone CS-56, purified from hybridoma cell culture
Synonym(s):
CSPG, chondroitin sulfate proteoglycan
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Check Cart for Availability | $194.00 |
100 μL | Check Cart for Availability | $533.00 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
CS-56, monoclonal
Species reactivity:
mouse, chicken, bovine, human
Application:
IF, IHC
Citations:
10
biological source
mouse
Quality Segment
antibody form
purified from hybridoma cell culture, purified immunoglobulin
antibody product type
primary antibodies
clone
CS-56, monoclonal
form
buffered aqueous solution
species reactivity
mouse, chicken, bovine, human
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunofluorescence: 2.5-5 μg/mL using bovine BMGE cells, immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon cancer sections and Biotin/ExtrAvidin ®-Peroxidase staining system.
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CSPG4(1464)
General description
Monoclonal anti-chondroitin sulfate antibody, (mouse IgM isotype) is derived from the hybridoma CS56 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with Ventral membranes of chicken gizzard fibroblasts. This antibody recognizes chondroitin sulfate containing proteoglycans (CSPG) in bovine mammary gland epithelial (BMGE) cells and also in human, chicken and mouse fibroblasts or tissues.
Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) is encoded by the gene mapped to human chromosome 15q24.2-15q24.3. It is a growth-inhibitory chondroitin sulfate proteoglycan (CSPG) produced after spinal cord injury. It has a single glycosaminoglycan (GAG) chain. The protein belongs to the transmembrane chondroitin sulfate proteoglycans family.
Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) is encoded by the gene mapped to human chromosome 15q24.2-15q24.3. It is a growth-inhibitory chondroitin sulfate proteoglycan (CSPG) produced after spinal cord injury. It has a single glycosaminoglycan (GAG) chain. The protein belongs to the transmembrane chondroitin sulfate proteoglycans family.
Immunogen
Ventral membranes of chicken gizzard fibroblasts
Biochem/physiol Actions
Chondroitin sulfate proteoglycan 4 (CSPG4) or nerve/glial antigen 2 (NG2) stimulates the activation of integrins in dorsal root ganglion neurons. It also has a role in stimulating inflammatory cytokine expression in microglia. CSPG4 plays a vital role in brain repair. Overexpression of the gene leads to developmental delay in patients with trisomy of 15q24-qter. CSPG4 serves as a potential prognostic biomarker and therapeutic target in malignant cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
for continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation. Working dilution samples should be discarded if not used within 12 hours.
Other Notes
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description clone CS-56, purified from hybridoma cell culture | description clone 132.38, purified immunoglobulin, buffered aqueous solution | description clone CS-56, ascites fluid | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| Gene Information human ... CSPG4(1464) | Gene Information rat ... Cspg4(81651) | Gene Information human ... ACAN(176) | Gene Information human ... CSPG4(1464) |
| antibody form purified immunoglobulin, purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form ascites fluid | antibody form affinity isolated antibody |
| clone CS-56, monoclonal | clone 132.38, monoclonal | clone CS-56, monoclonal | clone polyclonal |
| species reactivity mouse, chicken, bovine, human | species reactivity rat | species reactivity chicken, bovine, mouse, human, pig, rabbit, rat | species reactivity human |
| technique(s) immunofluorescence: 2.5-5 μg/mL using bovine BMGE cells, immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human colon cancer sections and Biotin/ExtrAvidin ®-Peroxidase staining system. | technique(s) western blot: 1 μg/mL using rat brain neonatal extract, indirect ELISA: suitable, immunoprecipitation (IP): suitable, immunocytochemistry: suitable, microarray: suitable | technique(s) indirect immunofluorescence: 1:200 using bovine mammary gland epithelial (BMGE) cells, microarray: suitable | technique(s) immunohistochemistry: 1:50- 1:200 |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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