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Anti-Involucrin antibody, Mouse monoclonal

Name: Anti-Involucrin antibody, Mouse monoclonal
Brand: SIGMA
Price: 534 USD
Availability: InStock

clone SY5, purified from hybridoma cell culture

Synonym(s):

Anti-IVL

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$534.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

SY5, monoclonal

Application:

IF, IHC, IP

Citations:

$534.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SY5, monoclonal

form

buffered aqueous solution

mol wt

~68 kDa

species reactivity

canine, gorilla, porcine, mouse, human, owl monkey

concentration

~1 mg/mL

technique(s)

immunoblotting: suitable, immunofluorescence: suitable, immunohistochemistry: 2.5-5 μg/mL using human skin sections, immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P07476

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IVL(3713)

Description

General description

Monoclonal Anti-Involucrin (mouse IgG1 isotype) is derived from the SY5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human involucrin. Involucrin is a soluble cytoplasmic protein. It is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer.

The skin serves as a vital barrier that protects the internal environment from external influences. As cells start to migrate out of the basal cell layer of the epidermis towards the surface of the skin, it lose the ability to divide and progress towards a terminal differentiation stage. During this process, keratinocytes go through marked morphological and structural changes. At the same time, the synthesis of important differentiation-dependent structural and catalytic proteins is initiated, which include keratins, filaggrin, transglutaminase, loricrin and involucrin. Involucrin (IVL) is a soluble cytoplasmic protein precursor of the epidermal cornified envelope that becomes crosslinked by transglutaminase during envelope assembly. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. It serves as an early marker of keratinocyte differentiation and a component of the cornified envelope. It is specifically expressed in the suprabasal layers of the epidermis. Involucrin accumulates in the spinous and granular layers as a non-crosslinked precursor and becomes covalently crosslinked to other proteins and lipids to form the cornified envelope scaffolding during the final stages of keratinocyte differentiation. Cell that have lost their ability to divide still continue to express Involucrin.

In pathological conditions, involucrin expression may be altered. In psoriasis and other benign epidermal hyperplasias, involucrin expression appears closer to the basal layer than it′s normal expression. In squamous cell carcinomas and premalignant lesions, involucrin is abnormally increases and found to be reduced in severe dysplasias of the larynx and cervix.

Monoclonal Anti-Involucrin specifically recognizes human and mouse Involucrin. The antibody does not react with mouse epidermis, thus enabling the use of the antibody for study of human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies.

Immunogen

purified human involucrin

Application

Anti-Involucrin antibody, Mouse monoclonal is recommended to use in various immunological techniques, including:

Immunoblot
Immunofluorescence
Immunohistochemistry
Immunoprecipitation

Biochem/physiol Actions

Involucrin (IVL) acts as a precursor of the epidermal cornified envelope that becomes crosslinked by transglutaminase during envelope assembly. Involucrin accumulates in the spinous and granular layers as a non-crosslinked precursor and becomes covalently crosslinked to other proteins and lipids to form the cornified envelope scaffolding during the final stages of keratinocyte differentiation. Cells that have lost their ability to divide express involucrin. In pathological conditions, involucrin expression may be altered. In psoriasis and other benign epidermal hyperplasias, involucrin expression appears closer to the basal layer than its normal expression. In squamous cell carcinomas and premalignant lesions, involucrin is abnormally increased and found to be reduced in severe dysplasias of the larynx and cervix.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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This Item	SAB4501594	SAB4200732	SAB4200273
Sigma-Aldrich SAB4200794 Anti-Involucrin antibody, Mouse monoclonal	Sigma-Aldrich SAB4501594 Anti-Involucrin antibody produced in rabbit	Sigma-Aldrich SAB4200732 Anti-β-Tubulin I antibody, Mouse monoclonal	Sigma-Aldrich SAB4200273 Anti-Orai1 antibody, Mouse monoclonal
description clone SY5, purified from hybridoma cell culture	description affinity isolated antibody	description clone SAP.4G5, purified from hybridoma cell culture	description clone ORAI1-89, purified from hybridoma cell culture
species reactivity canine, gorilla, porcine, mouse, human, owl monkey	species reactivity human	species reactivity bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey	species reactivity human
biological source mouse	biological source rabbit	biological source mouse	biological source mouse
antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
clone SY5, monoclonal	clone polyclonal	clone SAP.4G5, monoclonal	clone ORAI1-89, monoclonal
Gene Information human ... IVL(3713)	Gene Information human ... IVL(3713)	Gene Information human ... TUBB(203068)	Gene Information human ... ORAI1(84876)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

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