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SDF-1 ALPHA human

Name: SDF-1 ALPHA human
Brand: SIGMA

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

CXCL12, PBSF

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352202

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Properties

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

potency

20.0-80.0 ng/mL

mol wt

8.0 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P48061

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CXCL12(6387)

Description

General description

SDF-1α (stromal cell-derived factor 1 α) and β are stromal derived CXC chemokines, and signal through the CXCR4 (C-X-C motif chemokine receptor). SDF1α is an isoform of CXCL12 (C-X-C motif chemokine 12, also referred to as SDF-1). The gene is mapped to human chromosome 10q11. Recombinant human SDF-1a is an 8.0kDa protein containing 68 amino acid residues.

Application

SDF-1α (stromal cell-derived factor 1 α) human has been used to study the role of SDF-1α in cell migration and survival after TGF (transforming growth factor)-β-induced EMT (epithelial–mesenchymal transition) in hepatoma cells. It has been used to study podia formation, migration, proliferation and cell-cell adhesion of human hematopoietic progenitor cells in presence of SDF-1α.

SDF-1α (stromal cell-derived factor 1 α) human has been used in the chemotaxis assay of neural crest cells (NCCs) and as a component of Dulbecco′s modified Eagle′s medium (DMEM) in migration assay of human mesenchymal stem cells (hMSCs).

Biochem/physiol Actions

SDF-1α (stromal cell-derived factor 1 α) and β chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. SDF-1 is mainly expressed in the inflamed and failing myocardium. It is involved in inflammation, proliferation, tumorigenicity and metastasis. The SDF-1 proteins exert HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4). It is upregulated in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 is also involved in neuroprotection after ischemic stroke with the help of CXCR4.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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This Item	SRP3277	S8406	SRP3253
Sigma-Aldrich SRP3276 SDF-1 ALPHA human	Sigma-Aldrich SRP3277 SDF-1 BETA human	Sigma-Aldrich S8406 Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human	Sigma-Aldrich SRP3253 SDF-1 beta from rat
description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)	description >97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture	description recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay >97% (SDS-PAGE)	assay ≥95% (HPLC), ≥95% (SDS-PAGE)
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
biological source human	biological source human	biological source human	biological source rat
shipped in wet ice	shipped in wet ice	shipped in -	shipped in wet ice
UniProt accession no. P48061	UniProt accession no. P48061	UniProt accession no. P48061	UniProt accession no. Q9QZD1
mol wt 8.0 kDa	mol wt 8.5 kDa	mol wt ~8 kDa	mol wt 8.4 kDa

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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