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Anti-U2AF⁶⁵ antibody, Mouse monoclonal

Name: Anti-U2AF<SUP>65</SUP> antibody, Mouse monoclonal
Brand: SIGMA
Price: 201 USD
Availability: InStock

clone MC3, purified from hybridoma cell culture

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$201.00
100 μL	Check Cart for Availability	$604.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03456068

Conjugate:

unconjugated

Clone:

MC3, monoclonal

Application:

ARR, ELISA (i), EM, ICC, IHC, IP, WB

Citations:

$201.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC3, monoclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, rat, Xenopus, mouse

packaging

antibody small pack of 25 μL

technique(s)

electron microscopy: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells

isotype

IgG2b

UniProt accession no.

P26368

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... U2AF2(11338)
mouse ... U2af2(22185)
rat ... U2af2(308335)

Description

General description

Monoclonal Anti-U2AF⁶⁵ (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF⁶⁵. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF⁶⁵kDa subunit is a non-snRNP protein component of spliceosome complexes.

Immunogen

recombinant human U2AF⁶⁵.

Application

Monoclonal Anti-U2AF⁶⁵antibody produced in mouse has been used in:

RNA immunoprecipitation
immunoblotting
chromatin immunoprecipitation (ChIP)
enzyme-linked immunosorbent assay (ELISA)
immunohistochemistry
immunocytochemistry
immunoelectron microscopy

Biochem/physiol Actions

U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF⁶⁵ primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF⁶⁵) and (U2AF³⁵) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R4528	SAB1402953	HPA043562
Sigma-Aldrich U4758 Anti-U2AF⁶⁵ antibody, Mouse monoclonal	Sigma-Aldrich R4528 Anti-hnRNP-A1 antibody, Mouse monoclonal	Sigma-Aldrich SAB1402953 Monoclonal Anti-U2AF1 antibody produced in mouse	Sigma-Aldrich HPA043562 Anti-U2AF2 antibody produced in rabbit
description clone MC3, purified from hybridoma cell culture	description clone 9H10, purified from hybridoma cell culture	description clone 1C8, purified immunoglobulin, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Gene Information human ... U2AF2(11338) mouse ... U2af2(22185) rat ... U2af2(308335)	Gene Information human ... HNRNPA1(3178) mouse ... Hnrnpa1(15382) rat ... Hnrnpa1(29578)	Gene Information human ... U2AF1(7307)	Gene Information human ... U2AF2(11338)
clone MC3, monoclonal	clone 9H10, monoclonal	clone 1C8, monoclonal	clone polyclonal
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
species reactivity human, rat, Xenopus, mouse	species reactivity human, hamster, monkey, rat, bovine, mouse, canine	species reactivity human	species reactivity human

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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