Key Documents

View All Documentation

Anti-Ubiquitin antibody produced in rabbit

Name: Anti-Ubiquitin antibody produced in rabbit
Brand: SIGMA
Price: 598 USD
Availability: InStock

whole antiserum, lyophilized powder

Synonym(s):

Anti-Ubiquitin antibody

Select a Size

Change View

Size/SKU	Availability	Price
1 vial	Available to ship TODAYfromMILWAUKEE	$598.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162897

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC (f), WB

Citations:

121

$598.00

Available to ship TODAYDetails

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

mol wt

antigen 8.6 kDa

species reactivity

prokaryotes, eukaryotes

technique(s)

immunohistochemistry (frozen sections): suitable, western blot: 1:100 using ubiquitin purified from bovine red blood cells

UniProt accession no.

P0CG47

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)

Description

General description

Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2.

Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes.

Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.

Immunogen

bovine red blood cell ubiquitin.

Application

Anti-Ubiquitin antibody produced in rabbit is used in:

immunostaining in neuropathology
immunohistochemistry
western blotting

Biochem/physiol Actions

By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.

Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis.

Physical form

Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.

Preparation Note

Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	SAB4503053	MABS486	662099
Sigma-Aldrich U5379 Anti-Ubiquitin antibody produced in rabbit	Sigma-Aldrich SAB4503053 Anti-Ubiquitin antibody produced in rabbit	Sigma-Aldrich MABS486 Anti-Ubiquitin (pan) Antibody, UbiSite^™ ,clone 2G7B8	Sigma-Aldrich 662099 Anti-Ubiquitin Rabbit pAb
description whole antiserum, lyophilized powder	description affinity isolated antibody	description clone 2G7B8, from mouse	description liquid, Calbiochem^®
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
antibody form whole antiserum	antibody form affinity isolated antibody	antibody form purified antibody	antibody form serum
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level 100
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
technique(s) western blot: 1:100 using ubiquitin purified from bovine red blood cells, immunohistochemistry (frozen sections): suitable	technique(s) ELISA: 1:20000, immunohistochemistry: 1:50-1:100	technique(s) western blot: suitable	technique(s) -
clone polyclonal	clone polyclonal	clone 2G7B8, monoclonal	clone polyclonal

Still not finding the right product?

Explore all of our products under Anti-Ubiquitin antibody produced in rabbit

— or —

Try our Product Selector Tool to narrow your options

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.