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U7757

Anti-UCP-3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SLC25A9

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Size/SKUAvailabilityPrice
0.2 mL

Available to ship TODAYfromMILWAUKEE

$556.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03097017
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC (p), WB
Citations:
16

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~34 kDa

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using protease-digested sections of human and mouse skeletal muscle., western blot: 1:1,000 using extract of E. coli expressing UCP3

UniProt accession no.

P55916

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UCP3(7352)
mouse ... Ucp3(22229)
rat ... Ucp3(25708)

General description

Anti-Uncoupling Protein 3 (UCP3) is developed in rabbit using a synthetic peptide corresponding to amino acid residues 254-267 of human UCP3, with N-terminal lysine added, conjugated to keyhole limpet hemocyanin (KLH). The uncoupling proteins (UCPs) are mitochondrial inner membrane proteins which comprises UCP1, 2, 3, 4 and 5. UCP3 displays two forms, a long mature, UCP3L and a short, UCP3S form. UCP3 is preferentially expressed in skeletal muscle and in brown adipose tissue (BAT).
UCP3 is a skeletal muscle protein that functions to uncouple mitochondrial respiration from ATP production. UCP3 regulates fatty acid and energy metabolism. This protein inhibits lipid-induced oxidative stress in the mitochondrial components of heart and skeletal muscle cells. UCP3 may also be involved in the pathogenesis of type 2 diabetes . Anti-UCP-3 antibody is specific for UCP3 in humans, rats and mice and does not react with UCP1 and UCP2. Staining of the UCP3 band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

The corresponding sequence in rat and mouse UCP3 differ by 4 and 3 amino acids, respectively.
synthetic peptide corresponding to human UCP-3 sequence (amino acids 254-267) with N-terminal lysine added, conjugated to KLH.

Application

Anti-UCP-3 antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections at 1:3,000 dilution, using protease-digested sections of human and mouse skeletal muscle). The antibody may also be used in immunoblot (approx. 34 kDa, additional weak bands may be detected in some preparations of brown adipose tissue extracts) and western blot (at 1:1,000 dilution, extract of E. coli expressing UCP3).
Anti-UCP-3 antibody produced in rabbit has been used in immunohistochemistry and western blotting.

Biochem/physiol Actions

The uncoupling proteins (UCPs) are considered as transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They are capable of returning protons pumped by the respiratory chain to the mitochondrial matrix. UCP3 is an active proton transporter, regulated by CoQ (ubiquinone), fatty acids or nucleotides.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-UCP1 antibody produced in mouse clone 4B7, purified immunoglobulin, buffered aqueous solution

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description

affinity isolated antibody, buffered aqueous solution

description

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody

description

clone 4B7, purified immunoglobulin, buffered aqueous solution

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... UCP3(7352)
mouse ... Ucp3(22229)
rat ... Ucp3(25708)

Gene Information

human ... MFN2(9927)
mouse ... Mfn2(170731)
rat ... Mfn2(64476)

Gene Information

human ... UCP3(7352)

Gene Information

human ... UCP1(7350)

technique(s)

western blot: 1:1,000 using extract of E. coli expressing UCP3, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using protease-digested sections of human and mouse skeletal muscle.

technique(s)

western blot (chemiluminescent): 0.5-1 μg/mL using extract of mouse and rat brain mitochondria, immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate

technique(s)

western blot: 1:500-1:2000 (Cell Lysate), immunohistochemistry: 1:50- 1:200

technique(s)

capture ELISA: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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