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Anti-ATG5 (N-terminal) antibody produced in rabbit

Name: Anti-ATG5 (N-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 638 USD
Availability: InStock

affinity isolated antibody, PBS solution

Synonym(s):

ATG5 Antibody - Anti-ATG5 (N-terminal) antibody produced in rabbit, Atg5 Antibody, Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog (S. cerevisiae), Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like

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Pack Size	SKU	Availability	Price
200 μL		Available to ship TODAYfromMILWAUKEE	$638.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD09753847

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

$638.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

PBS solution

mol wt

antigen ~56 kDa (Atg5-Atg12 complex)

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

technique(s)

western blot: 0.5-1 μg/mL using whole extracts of human K562, rat NRK, and mouse 3T3 cells, immunofluorescence: suitable

UniProt accession no.

Q9H1Y0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)

Description

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-ATG5 (N-terminal) antibody has been used:

in immunoblotting
in immunofluorescence
in western blotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.

Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.

Biochem/physiol Actions

Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.

Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	A0731	A2859	A3606
Sigma-Aldrich A0856 Anti-ATG5 (N-terminal) antibody produced in rabbit	Sigma-Aldrich A0731 Anti-ATG5 (C-terminal) antibody produced in rabbit	Sigma-Aldrich A2859 Monoclonal Anti-ATG5 antibody produced in mouse	$Anti-ATG3 (C-terminal) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A3606 Anti-ATG3 (C-terminal) antibody produced in rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form IgG fraction of antiserum
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
form PBS solution	form -	form buffered aqueous solution	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000467683

0000086427

0000199537

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Global Trade Item Number

SKU	GTIN
A0856-200UL	04061833334539
A0856-25UL	04061837863370

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