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A0919

Mouse IgG−Agarose

(Suspension in 0.5 M NaCl containing preservative.)

Synonyme(s) :

IgG-Agarose Beads

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00164808
Conjugate:
agarose conjugate
Technique(s):
immunoprecipitation (IP): suitable
Application:
IP
Citations:
92

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conjugate

agarose conjugate

Quality Segment

200

antibody form

purified immunoglobulin

form

(Suspension in 0.5 M NaCl containing preservative.)

extent of labeling

1 mg per mL

technique(s)

immunoprecipitation (IP): suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases[1]. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice[2].

Application

IgG-Agarose can be used as an immunoadsorbent. It can also be used to affinity purify antibodies, remove species specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgGAgarose (resin volume). Mouse IgG-Agarose has been used for several immunoprecipitation assays[3],[4].
Mouse IgGs conjugated to agarose beads were used in immunoprecipitation assays to pre-clear HEK 293 whole cell extracts. Beads were incubated with extracts for 1 hour rotating at 4°C.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Cet article
A2909A7434A4416
Mouse IgG−Agarose (Suspension in 0.5 M NaCl containing preservative.)

Sigma-Aldrich

A0919

Mouse IgG−Agarose

Rabbit IgG−Agarose saline suspension

Sigma-Aldrich

A2909

Rabbit IgG−Agarose

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A7434

Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A4416

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody form

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

(Suspension in 0.5 M NaCl containing preservative.)

form

saline suspension

form

buffered aqueous glycerol solution

form

buffered aqueous solution

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well)

technique(s)

direct ELISA: 1:10,000

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Classe de stockage

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

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