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Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal

Name: Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal
Brand: SIGMA
Price: 568 USD
Availability: InStock

clone DI-22, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Digoxin, Monoclonal Anti-Digoxin–Alkaline Phosphatase antibody produced in mouse

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$568.00
0.5 mL	Check Cart for Availability	$802.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

alkaline phosphatase conjugate

Clone:

DI-22, monoclonal

Application:

DB, ELISA (d)

Citations:

$568.00

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Properties

biological source

mouse

Quality Segment

200

recombinant

expressed in mouse cell line

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DI-22, monoclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate, direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate, dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Digoxin is a cardiac glycoside that is used to treat chronic heart failure.Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody can be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies and peptides. The antibody is specific for digoxin and digoxin-labeled compounds. The product shows strong cross-reactivity with digoxigenin.

Immunogen

Digoxin-KLH.

Application

Monoclonal Anti-Digoxin-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:10000-1:6000) and dot blot (at 1:20,000 dilutions using digoxigenin-labeled biomolecules). It has also been used for enzyme immunoassay (EIA).

Nested PCR products were hybridized with 5′-end digoxigenin-labeled oligonucleotide probe. ELISA assays were performed to detect the probe by adding monoclonal anti-digoxin antibody alkaline phosphatase (DI-22) diluted 1:1000 in 1 M phosphate-buffered saline, 1% bovine serum albumin, and 0.1% Tween 20 (Ph 7.4). Detection was performed using Sigma Fass-Pnpp substrate tablets.

Biochem/physiol Actions

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0 containing 1% BSA, 50% glycerol, 1 mM MgCl₂ and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200669	D8156	A3076
Sigma-Aldrich A1054 Anti-Digoxin–Alkaline Phosphatase antibody, Mouse monoclonal	Sigma-Aldrich SAB4200669 Anti-Digoxin antibody, Mouse monoclonal	Sigma-Aldrich D8156 Monoclonal Anti-Digoxin antibody produced in mouse	Sigma-Aldrich A3076 Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal
description clone DI-22, purified from hybridoma cell culture	description clone DI-22, purified from hybridoma cell culture	description clone DI-22, ascites fluid	description clone GE-1, purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone DI-22, monoclonal	clone DI-22, monoclonal	clone DI-22, monoclonal	clone GE-1, monoclonal
conjugate alkaline phosphatase conjugate	conjugate -	conjugate unconjugated	conjugate alkaline phosphatase conjugate
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified from hybridoma cell culture
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
shipped in wet ice	shipped in dry ice	shipped in dry ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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