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Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse

Name: Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse
Brand: SIGMA
Price: 614 USD
Availability: InStock

clone PT-66, purified immunoglobulin, PBS solution

Synonym(s):

Phospho-Tyr, Monoclonal Anti-Phosphotyrosine, Phospho-tyrosine, p-Tyr

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Size/SKU	Availability	Price
0.5 mL	Estimated to ship TODAYfromSAINT LOUIS	$614.00
1 mL	Available to ship TODAYfromMILWAUKEE	$1,050.00

About This Item

NACRES:

NA.44

UNSPSC Code:

12352203

Conjugate:

agarose conjugate

Clone:

PT-66, monoclonal

Application:

Citations:

$614.00

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Properties

biological source

mouse

Quality Segment

200

recombinant

expressed in mouse cell line

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PT-66, monoclonal

form

PBS solution

technique(s)

immunoprecipitation (IP): suitable

isotype

IgG1

capacity

1 mg/mL binding capacity

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

phosphorylation (pTyr)

Description

General description

As determined by ELISA and competitive ELISA, the antibody reacts specifically with phosphorylated tyrosine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated tyrosine, phosphothreonine, phosphoserine, AMP or ATP.

Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

phosphotyrosine conjugated to BSA

Application

Monoclonal Anti-Phosphotyrosine-Agarose antibody produced in mouse has been used in:

immunoprecipitation experiments for affinity-purification of phosphotyrosine proteins
phosphotyrosine pulldown assays
PI3-kinase assay

Proteins containing phosphotyrosines were deteced in protein extracts from dissected tissue of Harlan Sprague Dawley rats or from transfected HEK293 cells using mouse monoclonal anti-phosphotyrosine antibody as the primary antibody. Reactivity was blocked when extracts were previously treated with 10 mM phosphotyrosine but not 10 mM tyrosine showing the specifity of the antibody for phosphorylated tyrosines. Immunoprecipitation of proteins containing phosphorylated tyrosines in rat dorsat root ganglion homogenates was performed using mouse monoclonal anti-phosphotyrosine. The antibody was incubated with the homogenates in the presence of 50 mm NaF and protease inhibitors for 4-14 hours at 4°. The antibody was precipitated using Protein G-agarose beads incubated overnight.

Biochem/physiol Actions

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Tyrosine-specific protein kinase activity has also been described in many retroviral oncogene proteins. Cells transformed by these oncogenes contain elevated levels of phosphotyrosine. Many of the oncogenes found in mammalian oncogenic viruses encode tyrosine protein kinases that reside in the cellular cytoplasm. Others encode transmembrane receptors whose tyrosine phosphotransferase activity is stimulated by the binding of ligand to the extracellular domain.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A5964	P5872	P6623
Sigma-Aldrich A1806 Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse	Sigma-Aldrich A5964 Monoclonal Anti-Phosphotyrosine–Peroxidase antibody produced in mouse	Sigma-Aldrich P5872 Anti-Phosphotyrosine antibody, Mouse monoclonal	Sigma-Aldrich P6623 Anti-Phosphothreonine antibody, Mouse monoclonal
description clone PT-66, purified immunoglobulin, PBS solution	description clone PT-66, purified immunoglobulin, lyophilized powder	description clone PT-66, purified from hybridoma cell culture	description clone PTR-8, purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone PT-66, monoclonal	clone PT-66, monoclonal	clone PT-66, monoclonal	clone PTR-8, monoclonal
conjugate agarose conjugate	conjugate peroxidase conjugate	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
technique(s) immunoprecipitation (IP): suitable	technique(s) direct ELISA: 1:60,000 using Phosphotyrosine-BSA, dot blot: 1:40,000-1:200,000 using phosphotyrosine-BSA using chromogenic and chemiluminescent substrates, respectively	technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets	technique(s) dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1 μg/mL, microarray: suitable, western blot: 5-10 μg/mL using A431 cell extracts
shipped in wet ice	shipped in -	shipped in dry ice	shipped in dry ice

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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