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Photobiotin acetate salt

For labeling DNA probes for hybridizations

Synonym(s):

Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt

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Size/SKUAvailabilityPrice
1 mg

Available to ship TODAYfromMILWAUKEE

$659.00
2 mg

Available to ship TODAYfromMILWAUKEE

$1,110.00

About This Item

Empirical Formula (Hill Notation):
C23H35N9O4S · C2H4O2
CAS Number:
Molecular Weight:
593.70
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:

$659.00


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grade

Molecular Biology

Quality Segment

assay

≥95%

form

film or powder

solubility

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

storage temp.

−20°C

SMILES string

CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]

InChI

1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1

InChI key

FFBLNTOMOSLSQM-AYEYRVMASA-N

General description

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Biochem/physiol Actions

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.
Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Disclaimer

Light sensitive.

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This Item
PHL898663871956448
description

For labeling DNA probes for hybridizations

description

phyproof® Reference Substance

description

suitable for microbiology, NutriSelect® Plus

description

analytical standard

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

100

assay

≥95%

assay

≥95.0% (HPLC)

assay

-

assay

≥98% (GC)

form

film or powder

form

solid

form

powder

form

-

solubility

H2O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

grade

Molecular Biology

grade

primary reference standard

grade

-

grade

analytical standard


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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