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A274

Monoclonal Anti-H+/K+ ATPase (β Subunit) antibody produced in mouse

clone 2G11, ascites fluid, buffered aqueous solution

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A voi/SKUDisponibilitàPrezzo
100 μL
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CHF 688.00

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD02096517
Conjugate:
unconjugated
Clone:
2G11, monoclonal
Application:
IF, IHC (f), WB CL
Citations:
4

CHF 688.00

Per conoscere la disponibilità, visualizza il carrello
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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2G11, monoclonal

form

buffered aqueous solution

mol wt

antigen 60-80 kDa (glycosylated form)

species reactivity

pig, mouse, rabbit, rat, bovine, ferret, canine

technique(s)

immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:2,000, western blot (chemiluminescent): 1:4,000

isotype

IgG1

UniProt accession no.

P18597

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Atp4b(11945)
rat ... Atp4b(24217)

General description

The H+/K+-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H+/K+-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H+/K+ ATPase (β subunit) binds to H+/K+-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.
The antibody reacts with various forms of the H+/K+ ATPase β subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the β subunit. The antibody has been shown to inhibit the enzymatic activity of the H+/K+-ATPase and to alter the affinity of the cytoplasmic K+ binding site. It may be used to localize and detect H+/K+ ATPase β subunit.

Immunogen

core 34 kDa H+/K+ ATPase β subunit from deglycosylated pig gastric microsomes.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-H+/K+ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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A273A9728D218
Monoclonal Anti-H+/K+ ATPase (β Subunit) antibody produced in mouse clone 2G11, ascites fluid, buffered aqueous solution

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Monoclonal Anti-H+/K+ ATPase (β Subunit) antibody produced in mouse

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A273

Monoclonal Anti-Na+/K+ ATPase (α3 Subunit) antibody produced in mouse

Monoclonal Anti-ATP Synthase β antibody produced in mouse 1 mg/mL, clone 4.3E8.D10, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

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Monoclonal Anti-ATP Synthase β antibody produced in mouse

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse clone 1A, buffered aqueous solution

Sigma-Aldrich

D218

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse

biological source

mouse

biological source

mouse

biological source

mouse

biological source

-

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

clone

2G11, monoclonal

clone

XVIF9-G10, monoclonal

clone

4.3E8.D10, monoclonal

clone

1A, monoclonal

Gene Information

mouse ... Atp4b(11945)
rat ... Atp4b(24217)

Gene Information

human ... ATP1A3(478)
rat ... Atp1a3(24213)

Gene Information

human ... ATP5B(506)
mouse ... Atp5b(11947)
rat ... Atp5b(171374)

Gene Information

human ... CACNA1S(779)
mouse ... Cacna1s(12292)
rat ... Cacna1s(682930)

species reactivity

pig, mouse, rabbit, rat, bovine, ferret, canine

species reactivity

human, rat, monkey, sheep, rabbit, guinea pig, canine

species reactivity

human, mouse, rat

species reactivity

human (weakly), guinea pig, mouse, rat, rabbit

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wgk

WGK 1

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Not applicable

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Not applicable

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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