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A275

Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody produced in mouse

clone 9A-5, ascites fluid

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100 μL
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$809.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD01633896
Conjugate:
unconjugated
Clone:
9A-5, monoclonal
Application:
ELISA (i), IHC (f)
Citations:
12

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9A-5, monoclonal

mol wt

antigen ~110 kDa

species reactivity

rat, human, canine, avian

technique(s)

immunohistochemistry (frozen sections): 1:1,000, indirect ELISA: suitable

isotype

IgG1

UniProt accession no.

P05023

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATP1A1(476)
rat ... Atp1a1(24211)

General description

Epitope mapping studies indicate the antibody binds within the intracellular region at or near Asp-369 of the α subunit. Staining is consistent with the plasma membrane localization of Na+/K+ ATPase.
Mouse Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody localizes the Na+/ K+ ATPase α1 subunit in human, canine, rat and avian tissues.
Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.
The gene coding Na+,K+-ATPase is mapped to human chromosome 1p13.1, which is a plasma membrane ion transporter. It is ubiquitously expressed in all animals. The α subunit of the protein consists of 10 transmembrane helices, and three cytoplasmic domains A, N and P that serve as actuator, in nucleotide-binding and for phosphorylation, respectively.

Immunogen

rat kidney Na+/K+ ATPase.

Application

Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody produced in mouse has been used in immunohistochemistry.
Mouse Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody has been used for immunohistochemical assays. It has also been used for immunolabeling assays to quantify the expression of proteins involved in cellular ion transport. Furthermore, the product is suitable for use in indirect ELISA.

Biochem/physiol Actions

Na+,K+-ATPase facilitates the transport of Na+ and K+ ions across plasma membrane in an ATP-dependent manner. Therefore, it is responsible for maintaining ion homeostasis across the cell and controls cellular excitability in electrically active tissues. The Na+ potential established by Na+,K+-ATPase is required for the function of secondary active transporters. Variation in the gene mostly results in protein function alteration, otherwise in some cases its stability, expression or plasma membrane targeting property is affected. Mutation in the gene is known to cause a number of disease conditions including Familial Hemiplegic Migraine type 2, Rapid-onset Dystonia Parkinsonism and Alternating Hemiplegia of Childhood. Na+,K+-ATPase serves as a receptor for cardiotonic steroids, that play a vital role in triggering proinflammatory cytokine production, in chronic inflammatory diseases, such as atherosclerosis.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Monoclonal Anti-Na+/K+ ATPase (α1 Subunit) antibody produced in mouse clone 9A-5, ascites fluid

Sigma-Aldrich

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Sigma-Aldrich

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Monoclonal Anti-Na+/Ca2+ Exchanger antibody produced in mouse clone C2C12

Sigma-Aldrich

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Anti-Na+/K+-ATPase antibody produced in rabbit

Sigma-Aldrich

SAB5701012

Anti-Na+/K+-ATPase antibody produced in rabbit

description

clone 9A-5, ascites fluid

description

clone 4E3, purified immunoglobulin, buffered aqueous solution

description

clone C2C12

description

-

Gene Information

human ... ATP1A1(476)
rat ... Atp1a1(24211)

Gene Information

human ... ATP1B2(482)

Gene Information

human ... SLC8A1(6546)

Gene Information

human ... ATP1A1(476)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

9A-5, monoclonal

clone

4E3, monoclonal

clone

C2C12, monoclonal

clone

monoclonal

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

-

antibody form

-

species reactivity

rat, human, canine, avian

species reactivity

human

species reactivity

human, rat, canine, rabbit, guinea pig

species reactivity

rat, human, mouse

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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