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Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody produced in mouse

Name: Monoclonal Anti-Na<SUP>+</SUP>/K<SUP>+</SUP> ATPase (α1 Subunit) antibody produced in mouse
Brand: SIGMA

clone 9A-5, ascites fluid

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01633896

Conjugate:

unconjugated

Clone:

9A-5, monoclonal

Application:

ELISA (i), IHC (f)

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

9A-5, monoclonal

mol wt

antigen ~110 kDa

species reactivity

rat, human, canine, avian

technique(s)

immunohistochemistry (frozen sections): 1:1,000, indirect ELISA: suitable

isotype

IgG1

UniProt accession no.

P05023

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATP1A1(476)
rat ... Atp1a1(24211)

Description

General description

Epitope mapping studies indicate the antibody binds within the intracellular region at or near Asp-369 of the α subunit. Staining is consistent with the plasma membrane localization of Na⁺/K⁺ ATPase.

Mouse Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody localizes the Na⁺/ K⁺ ATPase α1 subunit in human, canine, rat and avian tissues.

Na⁺/K⁺ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.

The gene coding Na⁺,K⁺-ATPase is mapped to human chromosome 1p13.1, which is a plasma membrane ion transporter. It is ubiquitously expressed in all animals. The α subunit of the protein consists of 10 transmembrane helices, and three cytoplasmic domains A, N and P that serve as actuator, in nucleotide-binding and for phosphorylation, respectively.

Immunogen

rat kidney Na⁺/K⁺ ATPase.

Application

Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody produced in mouse has been used in immunohistochemistry.

Mouse Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody has been used for immunohistochemical assays. It has also been used for immunolabeling assays to quantify the expression of proteins involved in cellular ion transport. Furthermore, the product is suitable for use in indirect ELISA.

Biochem/physiol Actions

Na⁺,K⁺-ATPase facilitates the transport of Na⁺ and K⁺ ions across plasma membrane in an ATP-dependent manner. Therefore, it is responsible for maintaining ion homeostasis across the cell and controls cellular excitability in electrically active tissues. The Na⁺ potential established by Na⁺,K⁺-ATPase is required for the function of secondary active transporters. Variation in the gene mostly results in protein function alteration, otherwise in some cases its stability, expression or plasma membrane targeting property is affected. Mutation in the gene is known to cause a number of disease conditions including Familial Hemiplegic Migraine type 2, Rapid-onset Dystonia Parkinsonism and Alternating Hemiplegia of Childhood. Na⁺,K⁺-ATPase serves as a receptor for cardiotonic steroids, that play a vital role in triggering proinflammatory cytokine production, in chronic inflammatory diseases, such as atherosclerosis.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	A273	D219	N216
Sigma-Aldrich A275 Monoclonal Anti-Na⁺/K⁺ ATPase (α1 Subunit) antibody produced in mouse	Sigma-Aldrich A273 Monoclonal Anti-Na⁺/K⁺ ATPase (α3 Subunit) antibody produced in mouse	Sigma-Aldrich D219 Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse	Sigma-Aldrich N216 Monoclonal Anti-Na⁺/Ca²⁺ Exchanger antibody produced in mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form -
species reactivity rat, human, canine, avian	species reactivity human, rat, monkey, sheep, rabbit, guinea pig, canine	species reactivity rat, mouse, rabbit, guinea pig	species reactivity human, rat, canine, rabbit, guinea pig
clone 9A-5, monoclonal	clone XVIF9-G10, monoclonal	clone 20A, monoclonal	clone C2C12, monoclonal
Gene Information human ... ATP1A1(476) rat ... Atp1a1(24211)	Gene Information human ... ATP1A3(478) rat ... Atp1a3(24213)	Gene Information mouse ... Cacna2d1(12293) rat ... Cacna2d1(25399)	Gene Information human ... SLC8A1(6546)
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source mouse	biological source mouse	biological source mouse	biological source mouse

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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