This product is prepared by reaction of the diazotized form of Sulfanilamide with casein. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/768/303/a2765pis.pdf
A2765
Azocasein
protease substrate, chromogenic, powder
Synonym(s):
Sulfanilamide-azocasein
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| Size/SKU | Availability | Price |
|---|---|---|
1 g | Check Cart for Availability | $69.80 |
5 g | Check Cart for Availability | $138.00 |
10 g | Check Cart for Availability | $237.00 |
25 g | Check Cart for Availability | $469.00 |
About This Item
$69.80
Check Cart for Availability
Product Name
Azocasein, protease substrate
biological source
bovine
Quality Segment
form
powder
technique(s)
ligand binding assay: suitable
solubility
water: 5 mg/mL, clear, orange to very deep orange
ε (extinction coefficient)
≥25 at 440 nm in 0.1 M NaOH at 1%
storage temp.
2-8°C
General description
Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.
Application
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
Azocasein has been used as a substrate for determination of protease activity.
Other Notes
View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| description protease substrate | description lyophilized powder, protease, essentially free, ≥98% (agarose gel electrophoresis) | description prolyl aminopeptidase substrate | description ≥90% (HPLC) |
| solubility water: 5 mg/mL, clear, orange to very deep orange | solubility H2O: soluble 50 mg/mL | solubility - | solubility water: 20 mg/mL, clear, orange to very deep red |
| technique(s) ligand binding assay: suitable | technique(s) ligand binding assay: suitable | technique(s) ligand binding assay: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| form powder | form lyophilized powder | form powder | form powder or crystals |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| biological source bovine | biological source bovine | biological source - | biological source - |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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A2765 Sigma-Aldrich アゾカゼイン この製品に含まれるアゾ色素を標品として使用したいのですが、どの化合物になるでしょうか？
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