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A2909

Rabbit IgG−Agarose

saline suspension

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Synonym(s):

IgG agarose beads

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2 mL

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$216.00
5 mL

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$411.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00165677
Conjugate:
agarose conjugate
Technique(s):
immunoprecipitation (IP): suitable
Application:
IP
Citations:
49

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conjugate

agarose conjugate

Quality Segment

200

form

saline suspension

extent of labeling

≥5 mg per mL

technique(s)

immunoprecipitation (IP): suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

General description

IgGs are glycoprotein antibodies that modulate several immune responses. IgG-Agarose is an immunoadsorbent that can be used to purify antibodies, remove species specific cross-reacting antibodies, or remove contaminating antibodies from an antiserum preparation. Characteristically, cross-reacting antibodies may be removed from an antiserum preparation using an equal resin volume of IgG-Agarose. However, the resin to antiserum ratio will vary with individual applications. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively.

Application

Rabbit IgG-Agarose has been used for immunoprecipitation and affinity purification assays.
Rabbit IgG antibody (20 μl/ml) crosslinked to agarose beads was used to isolate RAT tagged proteins from whole cell extracts of 293T cells.Rabbit IgG crosslinked to agarose beads were used for purification of tagged protein from mammalian whole cell extracts at 0.5 to 1.0mg total protein to 10 μl of beads.

Physical form

suspension in 0.5 M NaCl containing preservative.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Rabbit IgG−Agarose saline suspension

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description

saline suspension

description

affinity isolated antibody, saline suspension

description

affinity isolated antibody, aqueous glycerol suspension, 5 nm (colloidal gold)

description

affinity isolated antibody, buffered aqueous solution

conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

gold conjugate

conjugate

alkaline phosphatase conjugate

form

saline suspension

form

saline suspension

form

aqueous glycerol suspension

form

buffered aqueous solution

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoelectrophoresis: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

matrix

cross-linked 4% beaded agarose

matrix

Cross-linked 4% beaded agarose

matrix

-

matrix

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

Storage Class

10 - Combustible liquids

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000572900
    0000434689
    0000497765
    0000497766
    0000561348

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