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A2909

Rabbit IgG−Agarose

saline suspension

Sinonimo/i:

IgG agarose beads

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A voi/SKUDisponibilitàPrezzo
2 mL
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183,00 €
5 mL
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366,00 €

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00165677
Conjugate:
agarose conjugate
Technique(s):
immunoprecipitation (IP): suitable
Application:
IP
Citations:
49

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conjugate

agarose conjugate

Quality Segment

200

form

saline suspension

extent of labeling

≥5 mg per mL

technique(s)

immunoprecipitation (IP): suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

General description

IgGs are glycoprotein antibodies that modulate several immune responses. IgG-Agarose is an immunoadsorbent that can be used to purify antibodies, remove species specific cross-reacting antibodies, or remove contaminating antibodies from an antiserum preparation. Characteristically, cross-reacting antibodies may be removed from an antiserum preparation using an equal resin volume of IgG-Agarose. However, the resin to antiserum ratio will vary with individual applications. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively.

Application

Rabbit IgG antibody (20 μl/ml) crosslinked to agarose beads was used to isolate RAT tagged proteins from whole cell extracts of 293T cells.Rabbit IgG crosslinked to agarose beads were used for purification of tagged protein from mammalian whole cell extracts at 0.5 to 1.0mg total protein to 10 μl of beads.
Rabbit IgG-Agarose has been used for immunoprecipitation[1] and affinity purification assays[2].

Physical form

suspension in 0.5 M NaCl containing preservative.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Questo articolo
A0919P8047A8914
Rabbit IgG−Agarose saline suspension

Sigma-Aldrich

A2909

Rabbit IgG−Agarose

Mouse IgG−Agarose (Suspension in 0.5 M NaCl containing preservative.)

Sigma-Aldrich

A0919

Mouse IgG−Agarose

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−R-Phycoerythrin antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

P8047

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−R-Phycoerythrin antibody produced in goat

Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat affinity isolated antibody, saline suspension

Sigma-Aldrich

A8914

Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat

conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

phycoerythrin (R-PE) conjugate

conjugate

agarose conjugate

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

saline suspension

form

(Suspension in 0.5 M NaCl containing preservative.)

form

buffered aqueous solution

form

saline suspension

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

direct immunofluorescence: 1:32

technique(s)

immunoelectrophoresis: suitable

extent of labeling

≥5 mg per mL

extent of labeling

1 mg per mL

extent of labeling

-

extent of labeling

~1 mg per mL

matrix activation

cyanogen bromide

matrix activation

cyanogen bromide

matrix activation

-

matrix activation

cyanogen bromide

Classe di stoccaggio

10 - Combustible liquids

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

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