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Anti-AcV5 antibody, Mouse monoclonal

Name: Anti-AcV5 antibody, Mouse monoclonal
Brand: SIGMA
Price: 163.2 USD
Availability: InStock

clone ACV5, purified from hybridoma cell culture

Synonym(s):

Anti-Baculovirus envelope gp64 protein

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$163.20
100 μL	Available to ship TODAYfromMILWAUKEE	$534.00
200 μL	Available to ship TODAYfromMILWAUKEE	$724.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705571

Conjugate:

unconjugated

Clone:

ACV5, monoclonal

Application:

DB, ICC, IP, WB

Citations:

$163.20

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ACV5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.5-1 μg/mL using extracts of Sf9 cells infected with baculovirus

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Anti-AcV5 antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the hybridoma ACV5 produced by the fusion of mouse myeloma cells and splenocytes from C57Bl/6 X Balb/c mice immunized with AcNPV extracellular nonoccluded virus (NOV). GP64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

gp64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. It is involved in involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types.

Immunogen

AcNPV extracellular nonocculeded virus (NOV).

Application

Anti-AcV5 antibody, Mouse monoclonal has been used in:

immunoblotting
immunoprecipitation
dot blot
immunocytochemistry

Proteins tagged with this AcV5 peptide are functional in both dicot and monocot plants and show no cross reactivity across many plant species. Epitope tagged fusion proteins are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. Anti-AcV5 antibody, Mouse monoclonal, clone ACV5 may be used in immunoblotting, immunoprecipitation, dot blot, and immunocytochemistry.

Biochem/physiol Actions

GP64 (AcV5) is involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types. AcV5 is a monoclonal antibody that recognizes a nine amino acid residue tag (SWKDASGWS) of baculovirus Autographa californica GP64 envelope fusion protein (efp).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A1231	P5998	SAB4200722
Sigma-Aldrich A2980 Anti-AcV5 antibody, Mouse monoclonal	Sigma-Aldrich A1231 Anti-Aurora-A Kinase antibody, Mouse monoclonal	Sigma-Aldrich P5998 Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse	Sigma-Aldrich SAB4200722 Anti-Band 3 antibody, Mouse monoclonal
description clone ACV5, purified from hybridoma cell culture	description clone 35C1, purified from hybridoma cell culture	description ~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution	description clone BIII-136, purified from hybridoma cell culture
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone ACV5, monoclonal	clone 35C1, monoclonal	clone 35-206, monoclonal	clone BIII-136, monoclonal
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
mol wt antigen ~58 kDa	mol wt antigen ~46 kDa	mol wt -	mol wt -
isotype IgG2b	isotype IgG2b	isotype IgG2b	isotype IgG2a

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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