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Anti-AcV5 antibody, Mouse monoclonal

Name: Anti-AcV5 antibody, Mouse monoclonal
Brand: SIGMA
Price: 1615 NOK

clone ACV5, purified from hybridoma cell culture

Synonym(s):

Anti-Baculovirus envelope gp64 protein

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Size/SKU	Availability	Price
25 μL	Please contact Customer Service for Availability	1 900,00 kr 1 615,00 kr
100 μL	Please contact Customer Service for Availability	4 510,00 kr
200 μL	Please contact Customer Service for Availability	6 380,00 kr

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705571

Conjugate:

unconjugated

Clone:

ACV5, monoclonal

Application:

DB, ICC, IP, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ACV5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 0.5-1 μg/mL using extracts of Sf9 cells infected with baculovirus

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Anti-AcV5 antibody, Mouse monoclonal, (mouse IgG2b isotype) is derived from the hybridoma ACV5 produced by the fusion of mouse myeloma cells and splenocytes from C57Bl/6 X Balb/c mice immunized with AcNPV extracellular nonoccluded virus (NOV). GP64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

gp64 (AcV5) is an envelope glycoprotein found in the baculovirus Autographa californica multiple nucleopolyhedrovirus. It is involved in involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types.

Immunogen

AcNPV extracellular nonocculeded virus (NOV).

Application

Anti-AcV5 antibody, Mouse monoclonal has been used in:

immunoblotting
immunoprecipitation
dot blot
immunocytochemistry

Proteins tagged with this AcV5 peptide are functional in both dicot and monocot plants and show no cross reactivity across many plant species. Epitope tagged fusion proteins are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. Anti-AcV5 antibody, Mouse monoclonal, clone ACV5 may be used in immunoblotting, immunoprecipitation, dot blot, and immunocytochemistry.

Biochem/physiol Actions

GP64 (AcV5) is involved in viral entry into cells by receptor-mediated endocytosis. Elevated expression of gp64 has been shown to enhance gene transfer in a variety of cell types. AcV5 is a monoclonal antibody that recognizes a nine amino acid residue tag (SWKDASGWS) of baculovirus Autographa californica GP64 envelope fusion protein (efp).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A2859	P6874	A1106
Sigma-Aldrich A2980 Anti-AcV5 antibody, Mouse monoclonal	Sigma-Aldrich A2859 Monoclonal Anti-ATG5 antibody produced in mouse	Sigma-Aldrich P6874 Anti-p53 antibody, Mouse monoclonal	Sigma-Aldrich A1106 Anti-ATM antibody,Mouse monoclonal
description clone ACV5, purified from hybridoma cell culture	description clone ATG5-18, purified immunoglobulin, buffered aqueous solution	description clone DO-1, purified from hybridoma cell culture	description clone MAT3-4G10/8, purified from hybridoma cell culture
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone ACV5, monoclonal	clone ATG5-18, monoclonal	clone DO-1, monoclonal	clone MAT3-4G10/8, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution	form PBS solution

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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