Product activity is reported in batch-specific Certificate of Analysis. Below is a link to a sample COfA.
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3306-VAR0000329568.pdf
A3306
α-Amylase, heat-stable
solution, for use in Total Dietary Fiber Assay, TDF-100A
Sustainability features
Synonym(s):
α-Amylase from Bacillus licheniformis, Heat-Stable Alpha-Amylase, Heat-stable enzyme, 1,4-α-D-Glucan-glucanohydrolase
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| Size/SKU | Availability | Price |
|---|---|---|
10 mL | Estimated to ship onSeptember 25, 2027fromMILWAUKEE | $115.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-560-9
NACRES:
NA.32
MDL number:
EC Number:
Specific activity:
20000—60000 U/mL
Biological source:
bacterial (Bacillus sp.)
biological source
bacterial (Bacillus sp.)
Quality Segment
form
solution
quality
for use in Total Dietary Fiber Assay, TDF-100A
specific activity
20000—60000 U/mL
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features
technique(s)
activity assay: suitable
suitability
suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
application(s)
food and beverages
storage temp.
2-8°C
General description
α-Amylase, an extracellular enzyme, occurs in many natural sources, including animals, plants, and notably in microorganisms, such as various Bacillus species. α-Amylase is well-known as a heat-stable alkaline enzyme.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for "Energy efficiency" and "waste prevention" when used in starch ethanol research. For more information see the article in biofiles.
α-Amylase (1,4-α-d-glucan glucohydrolase) is an endo-acting glucanase. It belongs to the glycoside hydrolase family 13 (GH13). α-Amylase from Bacillus licheniformis NCIB 6346 can maintain >98% of activity after 60 minutes at pH 6.2 at 85 °C.
Application
α-Amylase, heat-stable has been used:
- as part of the animal feed in the experimental design to study the effect of dietary fiber on reproductive performance of sows during gestation
- as a supplement to prepare simulated small intestinal fluid (SSIF) and validate the in vitro digestibility by comparing with endogenous small intestinal fluid (ESIF) on different feeds in ducks
- to perform an in vitro intestinal digestion using raspberry dietary fiber
Biochem/physiol Actions
α-Amylase breaks down starch into sugars, by hydrolysis of the a-(1→4) glucan linkages in polysaccharides of three or more a-(1→4) linked D-glucose units, without hydrolyzing the α-(1→6) bond. It results in the formation of oligosaccharides including maltose, glucose, and alpha limit dextrin.
α-Amylases are predominantly utilized in processes involving starch saccharification and liquefaction, often requiring high-temperature conditions. These enzymes find wide industrial applications across sectors including food, fermentation, textile, paper, detergent, and pharmaceutical industries. In the processed-food industry, amylases play a vital role in baking, brewing, digestive aid preparation, cake production, fruit juice processing, and starch syrup manufacturing. Apart from their role in generating fermentable compounds, α-amylases contribute to anti-staling effects in breadmaking and enhance the softness retention of baked goods, thereby extending the shelf life of these products.
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
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This Item | |||
|---|---|---|---|
| description solution, for use in Total Dietary Fiber Assay, TDF-100A | description Type XII-A, saline solution, ≥500 units/mg protein (biuret) | description Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein | description lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE) |
| biological source bacterial (Bacillus sp.) | biological source - | biological source - | biological source Bacillus licheniformis |
| specific activity 20000—60000 U/mL | specific activity ≥500 units/mg protein (biuret) | specific activity 1,000-3,000 units/mg protein | specific activity 500-1,500 units/mg protein |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| form solution | form saline solution | form lyophilized powder | form lyophilized powder |
| application(s) food and beverages | application(s) - | application(s) - | application(s) - |
| suitability suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification | suitability - | suitability - | suitability - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Good afternoon, Can I ask the enzyme activity for the alpha amylase which coded by A3306?Because there is no mentioned in the website.
1 answer-
Helpful?
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Can the powdered A1031 α-Amylase enzyme from human saliva be dissolved into a water solution, aliquoted, frozen, and thawed for use?
1 answer-
It is unlikely that alpha amylase can be dissolved in water and frozen for long-term storage. There is no information available for product A1031 that suggests the enzyme is stable when dissolved in water and frozen at -20°C.
Helpful?
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I need to know the concentration of a-amylase A3306, how many units per one ml?
1 answer-
The activity of this product is reported on a lot specific basis and is reported in the Certificate of Analysis. The activity specification range is 20,000 - 60,000 Un/mL. Please see the link below to review a sample Certificate:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3306-BULKSLCN4587.pdfHelpful?
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