Key Documents

View All Documentation

Anti-β-Actin−Peroxidase antibody, Mouse monoclonal

Name: Anti-β-Actin−Peroxidase antibody, Mouse monoclonal
Brand: SIGMA
Price: 857 CAD

clone AC-15, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Actin, Loading Control

Select a Size

Change View

Size/SKU	Availability	Price
200 μL	Please contact Customer Service for Availability	$857.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

AC-15, monoclonal

Application:

Citations:

1602

$857.00

Please contact Customer Service for Availability

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-15, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig

should not react with

Dictyostelium discoideum, Drosophila

technique(s)

western blot: 1:25,000-1:50,000 using cell extracts of human foreskin fibroblasts or chicken fibroblasts

isotype

IgG1

UniProt accession no.

P60709

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Description

General description

Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds as at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non muscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.

In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.

Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Application

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.

Monoclonal mouse anti-beta actin antibody is suitable as a loading control or primary antibody for western blot analysis. It has also been used in chemiluminescent detection.

Biochem/physiol Actions

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The six isoforms of actins are characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	A1978	A5441	A5316
Sigma-Aldrich A3854 Anti-β-Actin−Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A1978 Anti-β-Actin antibody, Mouse monoclonal	Sigma-Aldrich A5441 Monoclonal Anti-β-Actin antibody produced in mouse	Sigma-Aldrich A5316 Monoclonal Anti-β-Actin antibody produced in mouse
description clone AC-15, purified from hybridoma cell culture	description clone AC-15, purified from hybridoma cell culture	description clone AC-15, ascites fluid	description clone AC-74, ascites fluid
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate peroxidase conjugate	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form ascites fluid	antibody form ascites fluid
species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human
clone AC-15, monoclonal	clone AC-15, monoclonal	clone AC-15, monoclonal	clone AC-74, monoclonal

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

Rating Snapshot

Select a row below to filter reviews.

Average Customer Ratings

Overall, average rating value is 5 of 5.

Active Filters

1 Ratings-Only Review