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Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse

Name: Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse
Brand: SIGMA
Price: 1010 USD
Availability: InStock

clone MBP-17, purified immunoglobulin, lyophilized powder

Synonym(s):

Monoclonal Anti-Maltose Binding Protein, Anti-MBP

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1 vial	Check Cart for Availability	$1,010.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

MBP-17, monoclonal

Application:

Citations:

$1,010.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MBP-17, monoclonal

form

lyophilized powder

packaging

vial of 0.5 mL

technique(s)

western blot: 1:1,000 using purified, recombinant MBP

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the MBP-17 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified recombinant MBP fusion protein. Maltose Binding Protein (MBP) is a periplasmic protein, which is specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is produced from the pre-protein containing 26 amino acids, as signal peptide.

The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.

Immunogen

Purified, recombinant MBP fusion protein

Application

Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.

Monoclonal Anti-Maltose Binding Protein Peroxidase antibody produced in mouse has been used in:

immunoblotting
dot blot
enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.

Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. It has been reported that the addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

Physical form

Lyophilized in 0.01 M sodium phosphate buffered saline containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

The antibody conjugate should be reconstituted with 0.5 mL deionized water.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	M1321	SAB4200082	M6295
Sigma-Aldrich A4213 Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse	Sigma-Aldrich M1321 Monoclonal Anti-Maltose Binding Protein antibody produced in mouse	Sigma-Aldrich SAB4200082 Monoclonal Anti-Maltose Binding Protein (MBP) antibody produced in rat	Sigma-Aldrich M6295 Monoclonal Anti-Maltose Binding Protein antibody produced in mouse
description clone MBP-17, purified immunoglobulin, lyophilized powder	description clone MBP-17, purified immunoglobulin, buffered aqueous solution	description 1.0 mg/mL, clone MBP 7G4, purified immunoglobulin	description clone MBP-17, ascites fluid
conjugate peroxidase conjugate	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone MBP-17, monoclonal	clone MBP-17, monoclonal	clone MBP 7G4, monoclonal	clone MBP-17, monoclonal
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form ascites fluid
biological source mouse	biological source mouse	biological source rat	biological source mouse
technique(s) western blot: 1:1,000 using purified, recombinant MBP	technique(s) dot blot: suitable, indirect ELISA: suitable, western blot: 0.05-0.1 μg/mL using purified recombinant MBP.	technique(s) immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 0.1-0.2 μg/mL using MBP recombinant protein	technique(s) dot blot: suitable, indirect ELISA: suitable, western blot: 1:4,000 using purified, recombinant MBP
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c