A4213
Monoclonal Anti-Maltose Binding Protein−Peroxidase antibody produced in mouse
clone MBP-17, purified immunoglobulin, lyophilized powder
Synonym(s):
Monoclonal Anti-Maltose Binding Protein, Anti-MBP
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Check Cart for Availability | $1,010.00 |
About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
MBP-17, monoclonal
Application:
WB
Citations:
3
biological source
mouse
Quality Segment
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MBP-17, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
technique(s)
western blot: 1:1,000 using purified, recombinant MBP
isotype
IgG1
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the MBP-17 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified recombinant MBP fusion protein. Maltose Binding Protein (MBP) is a periplasmic protein, which is specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is produced from the pre-protein containing 26 amino acids, as signal peptide.
The antibody recognizes native as well as denatured-reduced forms of purified MBP and MBP fusion proteins.
Immunogen
Purified, recombinant MBP fusion protein
Application
Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.
Monoclonal Anti-Maltose Binding Protein Peroxidase antibody produced in mouse has been used in:
- immunoblotting
- dot blot
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.
Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. It has been reported that the addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.
Physical form
Lyophilized in 0.01 M sodium phosphate buffered saline containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
The antibody conjugate should be reconstituted with 0.5 mL deionized water.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate unconjugated | conjugate unconjugated |
| clone MBP-17, monoclonal | clone BN-34, monoclonal | clone MBP-17, monoclonal | clone MBP 7G4, monoclonal |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source mouse | biological source mouse | biological source mouse | biological source rat |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| isotype IgG1 | isotype IgG1 | isotype IgG1 | isotype IgG2a |
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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