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Monoclonal Anti-β₁ and β₂-Adaptins antibody produced in mouse

Name: Monoclonal Anti-β<SUB>1</SUB> and β<SUB>2</SUB>-Adaptins antibody produced in mouse
Brand: SIGMA
Price: 4938.5 SEK

clone 100/1, ascites fluid

Synonym(s):

Anti-AP-1 and AP-2

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Size/SKU	Availability	Price
0.2 mL	Please contact Customer Service for Availability	5 810,00 kr 4 938,50 kr

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00212883

Conjugate:

unconjugated

Clone:

100/1, monoclonal

Application:

ARR, IF, WB

Citations:

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

100/1, monoclonal

contains

15 mM sodium azide

species reactivity

human, bovine, rat

technique(s)

indirect immunofluorescence: suitable, microarray: suitable, western blot: 1:200 using rat brain extract

isotype

IgG1

UniProt accession no.

P63010, Q10567

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AP1B1(162), AP2B1(163)
rat ... Ap1b1(29663), Ap2b1(140670)

Description

General description

Monoclonal Anti- β1 and β2-Adaptins (AP-1 & AP-2)(mouse IgG1 isotype) is derived from the 100/1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptins AP-1 and AP-2 are mapped to human chromosome 22q12.2 and 17q12, respectively. AP-1 adaptor consists of four subunits. It includes two large adaptins (one each of γ/α/δ/ε and β1−4 , one medium adaptin (μ1−4), and one small adaptin (ς1−4. Similarly, the AP-2 is made up of two 100-110 kDa polypeptides, termed α and β2 (formerly β) subunits, F2 , and s2 subunit. α and β2 subunits (α and β 2).

Immunogen

AP-2 adaptor protein from bovine brain.

Application

Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.

Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse has been used in immunoblotting and immunohistology.

Mouse monoclonal anti-β1 and β2-adaptins can be used for western blot (1:200) assays using rat brain extract. The antibody is also suitable for use in indirect immunofluorescence and microarray analyses. Furthermore, monoclonal anti-β1/β2-adaptins have been used for double staining methods to detect clathrin-coated regions of the plasma membrane in rat brain cells.

Biochem/physiol Actions

β1and β2 adaptins are highly homologous which suggests that the β -type adaptor chains may mediate the interaction of their adaptors with the highly conserved clathrin molecule. Monoclonal antibodies reacting specifically against adaptor proteins are useful tools for studies on the intracellular distribution and structural relationship of adaptor complexes.

Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.

Physical form

The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	A4200	A4325	G2279
Sigma-Aldrich A4450 Monoclonal Anti-β₁ and β₂-Adaptins antibody produced in mouse	Sigma-Aldrich A4200 Monoclonal Anti-γ-Adaptin antibody produced in mouse	Sigma-Aldrich A4325 Monoclonal Anti-α-Adaptin antibody produced in mouse	Sigma-Aldrich G2279 Monoclonal Anti-β-COP antibody produced in mouse
Gene Information human ... AP1B1(162), AP2B1(163) rat ... Ap1b1(29663), Ap2b1(140670)	Gene Information human ... AP1G1(164) mouse ... Ap1g1(11765) rat ... Ap1g1(171494)	Gene Information human ... AP2A1(160) rat ... Ap2a1(308578)	Gene Information human ... COPB1(1315) rat ... Copb1(114023)
species reactivity human, bovine, rat	species reactivity bovine, human, monkey	species reactivity bovine, human, rat	species reactivity monkey, human, chicken, goose, rabbit, canine, bovine, kangaroo rat, rat, hamster
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone 100/1, monoclonal	clone 100/3, monoclonal	clone 100/2, monoclonal	clone M3A5, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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