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Anti-ARTS antibody, Mouse monoclonal

Name: Anti-ARTS antibody, Mouse monoclonal
Brand: SIGMA

clone ARTS51, purified from hybridoma cell culture

Synonym(s):

Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway, Monoclonal Anti-ARTS antibody produced in mouse

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03096625

Conjugate:

unconjugated

Clone:

ARTS51, monoclonal

Application:

ARR, ELISA (i), ICC, WB

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ARTS51, monoclonal

form

buffered aqueous solution

mol wt

antigen 32 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SEPT4(5414)

Description

General description

^1,2 ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers.

Anti-ARTS antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the ARTS51 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminus of human ARTS protein, conjugated to KLH. ARTS (Apoptosis Related protein in the TGF-β signaling pathway), is a novel protein involved in the apoptotic pathways of transforming growth factor-β (TGF-β). The protein is derived from the human septin H5/PNUTL2/CDCrel-2a/2b gene. ARTS consists a phosphate-binding site (P-loop) death domain, which is conserved in all septins and in many different classes of ATP/GTPases. It is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β.

Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. Originally found to be essential for apoptosis induced by transforming growth factor-β (TGF-β) ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. Mouse monoclonal anti-ARTS antibody recognizes an epitope within amino acids 247-266 of human ARTS molecule.

Immunogen

synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.

Application

Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads.

Monoclonal anti-ARTS antibody has been used for western blot assays. The antibody has also been used for immunostaining applications. Furthermore, the product is suitable for use in indirect ELISA microarray and immunocytochemistry assays.

Biochem/physiol Actions

This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)

This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P6749	A2228	C4618
Sigma-Aldrich A4471 Anti-ARTS antibody, Mouse monoclonal	Sigma-Aldrich P6749 Anti-p53 antibody, Mouse monoclonal	Sigma-Aldrich A2228 Monoclonal Anti-β-Actin antibody produced in mouse	Sigma-Aldrich C4618 Monoclonal Anti-Cathepsin L antibody produced in mouse
description clone ARTS51, purified from hybridoma cell culture	description clone PAb1801, purified from hybridoma cell culture	description clone AC-74, purified immunoglobulin, buffered aqueous solution	description clone CPL33/1, purified from hybridoma cell culture
Gene Information human ... SEPT4(5414)	Gene Information human ... TP53(7157)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... CTSL1(1514)
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
clone ARTS51, monoclonal	clone PAb1801, monoclonal	clone AC-74, monoclonal	clone CPL33/1, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
species reactivity human	species reactivity human	species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human	species reactivity human

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