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A4471

Anti-ARTS antibody, Mouse monoclonal

clone ARTS51, purified from hybridoma cell culture

Synonym(s):

Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway, Monoclonal Anti-ARTS antibody produced in mouse

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0.1 mL

Available to ship TODAYfromMILWAUKEE

$579.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03096625
Conjugate:
unconjugated
Clone:
ARTS51, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
11

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ARTS51, monoclonal

form

buffered aqueous solution

mol wt

antigen 32 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SEPT4(5414)

General description

1,2 ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers.
Anti-ARTS antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the ARTS51 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminus of human ARTS protein, conjugated to KLH. ARTS (Apoptosis Related protein in the TGF-β signaling pathway), is a novel protein involved in the apoptotic pathways of transforming growth factor-β (TGF-β). The protein is derived from the human septin H5/PNUTL2/CDCrel-2a/2b gene. ARTS consists a phosphate-binding site (P-loop) death domain, which is conserved in all septins and in many different classes of ATP/GTPases. It is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β.
Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. Originally found to be essential for apoptosis induced by transforming growth factor-β (TGF-β) ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. Mouse monoclonal anti-ARTS antibody recognizes an epitope within amino acids 247-266 of human ARTS molecule.

Immunogen

synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.

Application

Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads.
Monoclonal anti-ARTS antibody has been used for western blot assays. The antibody has also been used for immunostaining applications. Furthermore, the product is suitable for use in indirect ELISA microarray and immunocytochemistry assays.

Biochem/physiol Actions

This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)

This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-ARTS antibody, Mouse monoclonal clone ARTS51, purified from hybridoma cell culture

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Gene Information

human ... SEPT4(5414)

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Gene Information

human ... CTSL1(1514)

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

species reactivity

human

species reactivity

rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human

species reactivity

human

species reactivity

rat, chicken, human, mouse, bovine, quail

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

ARTS51, monoclonal

clone

AC-74, monoclonal

clone

CPL33/1, monoclonal

clone

HM-2, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000541672
0000541672
0000507335
0000507335
0000462323

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Related Content

Dana Mamriev et al.
Cell death & disease, 11(6), 483-483 (2020-06-27)
Many human cancers over-express B cell lymphoma 2 (Bcl-2) or X-linked inhibitor of apoptosis (IAP) proteins to evade cell death. The pro-apoptotic ARTS (Sept4_i2) protein binds directly to both Bcl-2 and XIAP and promotes apoptosis by stimulating their degradation via
Kristi L Bennett et al.
Cancer research, 69(24), 9301-9305 (2009-11-26)
Head and neck squamous cell carcinoma (HNSCC) is an aggressive cancer with low survival rates in advanced stages. To facilitate timely diagnosis and improve outcome, early detection markers (e.g., DNA methylation) are crucial for timely cancer diagnosis. In a recent
A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif
Larisch S, et al.
Nature Cell Biology, 2(12), 915-915 (2000)
View All Related Papers

Global Trade Item Number

SKUGTIN
A4471-.1ML04061838160690

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