Documenti fondamentali

Visualizza tutta la documentazione

Vai a

A4471

Anti-ARTS antibody, Mouse monoclonal

Name: Anti-ARTS antibody, Mouse monoclonal
Brand: SIGMA
Price: 506 EUR

clone ARTS51, purified from hybridoma cell culture

Sinonimo/i:

Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway, Monoclonal Anti-ARTS antibody produced in mouse

per visualizzare i prezzi organizzativi e contrattuali.

Scegli un formato

Cambia visualizzazione

A voi/SKU	Disponibilità	Prezzo
0.1 mL	Per informazioni sulla disponibilità, contatta il Servizio Clienti.	506,00 €

Informazioni su questo articolo

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03096625

Conjugate:

unconjugated

Clone:

ARTS51, monoclonal

Application:

ARR, ELISA (i), ICC, WB

Citations:

506,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Richiedi un ordine personalizzato

Servizio Tecnico

Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.

Permettici di aiutarti

Proprietà

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ARTS51, monoclonal

form

buffered aqueous solution

mol wt

antigen 32 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SEPT4(5414)

Descrizione

General description

^1,2 ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers.

Anti-ARTS antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the ARTS51 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminus of human ARTS protein, conjugated to KLH. ARTS (Apoptosis Related protein in the TGF-β signaling pathway), is a novel protein involved in the apoptotic pathways of transforming growth factor-β (TGF-β). The protein is derived from the human septin H5/PNUTL2/CDCrel-2a/2b gene. ARTS consists a phosphate-binding site (P-loop) death domain, which is conserved in all septins and in many different classes of ATP/GTPases. It is localized to mitochondria and translocates to the nucleus, coincident with apoptotic signals induced by TGF-β.

Apoptosis-related protein in TGF-β signaling pathway (ARTS) is a novel mitochondrial protein that mediates apoptosis induced by a variety of pro-apoptotic stimuli. Originally found to be essential for apoptosis induced by transforming growth factor-β (TGF-β) ARTS was later shown to be a key apoptotic protein mediating apoptosis initiated by other inducers. Mouse monoclonal anti-ARTS antibody recognizes an epitope within amino acids 247-266 of human ARTS molecule.

Immunogen

synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.

Application

Immunoprecipitation of COS-7 cell lysates was performed using 10 μl of monoclonal mouse anti-ARTS antibody. Antibody was allowed to bind for 4 hours on ice before precipitated using G-sepharose beads.

Monoclonal anti-ARTS antibody has been used for western blot assays. The antibody has also been used for immunostaining applications. Furthermore, the product is suitable for use in indirect ELISA microarray and immunocytochemistry assays.

Biochem/physiol Actions

This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)

This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Confronta articoli simili

Visualizza il confronto per intero

Mostra le differenze

1 of 1

Questo articolo	A2228	SAB4200501	A8353
Sigma-Aldrich A4471 Anti-ARTS antibody, Mouse monoclonal	Sigma-Aldrich A2228 Anti-actinaβ monoclonale	Sigma-Aldrich SAB4200501 Anti-SFPQ antibody, Mouse monoclonal	Sigma-Aldrich A8353 Anti-hABH3 antibody, Mouse monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity human	species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human	species reactivity rat, human, mouse	species reactivity rat, hamster, monkey, bovine, canine, human, mouse
clone ARTS51, monoclonal	clone AC-74, monoclonal	clone FC23, monoclonal	clone hABH3-99, monoclonal
Gene Information human ... SEPT4(5414)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... SFPQ(6421) mouse ... Sfpq(71514) rat ... Sfpq(252855)	Gene Information Monkey ... ALKBH3(715921) human ... ALKBH3(221120) mouse ... Alkbh3(69113) rat ... Alkbh3(362169)
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin

Still not finding the right product?

Provate il nostro Motore di ricerca dei prodotti per restringere le opzioni.

Classe di stoccaggio

12 - Non Combustible Liquids

wgk

nwg

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.