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A5044

Monoclonal Anti-α-Actinin antibody produced in mouse

clone BM-75.2, ascites fluid

Synonym(s):

Anti-BDPLT15

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100 μL
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$580.00
0.2 mL
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$727.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162107
Conjugate:
unconjugated
Clone:
BM-75.2, monoclonal
Application:
ARR, IF, WB
Citations:
133

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BM-75.2, monoclonal

mol wt

antigen 100 kDa

contains

15 mM sodium azide

species reactivity

mouse, bovine, human, chicken

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts, microarray: suitable, western blot: suitable

isotype

IgM

UniProt accession no.

P12814

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTN1(87)
mouse ... Actn1(109711)

General description

α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells.
The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-actinin belongs to the spectrin protein superfamily.

Immunogen

cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells.

Application

Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000.
May be used in the study of skeletal muscle organization and in studies of the membrane anchorage sites of actin.
Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays. It can also be used in western blot, microarray, immunohistochemistry and in Immunofluorescence microscopy.

Biochem/physiol Actions

α−actinin regulates muscle contraction and cytoskeletal organization. It promotes cell migration and acts as an actin crosslinker. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A2547A7811A7607
Monoclonal Anti-α-Actinin antibody produced in mouse clone BM-75.2, ascites fluid

Sigma-Aldrich

A5044

Monoclonal Anti-α-Actinin antibody produced in mouse

Monoclonal Anti-Actin, α-Smooth Muscle clone 1A4, ascites fluid

Sigma-Aldrich

A2547

Monoclonal Anti-Actin, α-Smooth Muscle

Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse clone EA-53, ascites fluid

Sigma-Aldrich

A7811

Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse ~1.0 mg/mL, clone CGA7, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

A7607

Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

species reactivity

mouse, bovine, human, chicken

species reactivity

human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake

species reactivity

mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard

species reactivity

rat, chicken, mouse, human, monkey

Gene Information

human ... ACTN1(87)
mouse ... Actn1(109711)

Gene Information

human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)

Gene Information

chicken ... ACTA2(423787)
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

BM-75.2, monoclonal

clone

1A4, monoclonal

clone

EA-53, monoclonal

clone

CGA7, monoclonal

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000363223
0000363222
0000324044
0000291299
0000363223

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Related Content

Thomas A Hawkins et al.
Development (Cambridge, England), 135(6), 1147-1156 (2008-02-08)
The mechanisms that regulate sarcomere assembly during myofibril formation are poorly understood. In this study, we characterise the zebrafish sloth(u45) mutant, in which the initial steps in sarcomere assembly take place, but thick filaments are absent and filamentous I-Z-I brushes
C M Laukaitis et al.
The Journal of cell biology, 153(7), 1427-1440 (2001-06-27)
To investigate the mechanisms by which adhesions form and disperse in migrating cells, we expressed alpha 5 integrin, alpha-actinin, and paxillin as green fluorescent protein (GFP) fusions. All localized with their endogenous counterparts and did not perturb migration when expressed
C A Conley
American journal of physiology. Cell physiology, 280(6), C1645-C1656 (2001-05-15)
Evidence is accumulating to suggest that actin filament remodeling is critical for smooth muscle contraction, which implicates actin filament ends as important sites for regulation of contraction. Tropomodulin (Tmod) and smooth muscle leiomodin (SM-Lmod) have been found in many tissues
View All Related Papers

Global Trade Item Number

SKUGTIN
A5044-.2ML04061837718854
A5044-100UL04061837718861

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