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Monoclonal Anti-α-Actinin antibody produced in mouse

Name: Monoclonal Anti-α-Actinin antibody produced in mouse
Brand: SIGMA
Price: 580 USD
Availability: InStock

clone BM-75.2, ascites fluid

Synonym(s):

Anti-BDPLT15

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Size/SKU	Availability	Price
100 μL	Available to ship onJuly 22, 2026fromMILWAUKEE	$580.00
0.2 mL	Available to ship onJuly 22, 2026fromMILWAUKEE	$727.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162107

Conjugate:

unconjugated

Clone:

BM-75.2, monoclonal

Application:

ARR, IF, WB

Citations:

133

$580.00

Available to ship onJuly 22, 2026Details

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BM-75.2, monoclonal

mol wt

antigen 100 kDa

contains

15 mM sodium azide

species reactivity

mouse, bovine, human, chicken

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts, microarray: suitable, western blot: suitable

isotype

IgM

UniProt accession no.

P12814

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTN1(87)
mouse ... Actn1(109711)

Description

General description

α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. The product has also shown reactivity for mouse and human cells.

The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-actinin belongs to the spectrin protein superfamily.

Immunogen

cytoskeletal fraction of bovine mammary gland epithelial (BMGE) cells.

Application

Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000.

May be used in the study of skeletal muscle organization and in studies of the membrane anchorage sites of actin.

Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays. It can also be used in western blot, microarray, immunohistochemistry and in Immunofluorescence microscopy.

Biochem/physiol Actions

α−actinin regulates muscle contraction and cytoskeletal organization. It promotes cell migration and acts as an actin crosslinker. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A7811	A5441	A2172
Sigma-Aldrich A5044 Monoclonal Anti-α-Actinin antibody produced in mouse	Sigma-Aldrich A7811 Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse	Sigma-Aldrich A5441 Monoclonal Anti-β-Actin antibody produced in mouse	Sigma-Aldrich A2172 Monoclonal Anti-Actin (α-Sarcomeric) antibody produced in mouse
description clone BM-75.2, ascites fluid	description clone EA-53, ascites fluid	description clone AC-15, ascites fluid	description clone 5C5, ascites fluid
antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid	antibody form ascites fluid
species reactivity mouse, bovine, human, chicken	species reactivity mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity rabbit, carp, rat, sheep, guinea pig, human, snake, bovine, frog
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone BM-75.2, monoclonal	clone EA-53, monoclonal	clone AC-15, monoclonal	clone 5C5, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Gene Information human ... ACTN1(87) mouse ... Actn1(109711)	Gene Information human ... ACTN2(88) mouse ... Actn2(11472) rat ... Actn2(291245)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTA1(58), ACTC1(70) mouse ... Rwdd2a(69519) rat ... Acta1(29437), Actc1(29275)

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c