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Anti-ARF6 antibody, Mouse monoclonal

Name: Anti-ARF6 antibody, Mouse monoclonal
Brand: SIGMA
Price: 650 USD
Availability: InStock

clone ARFAG, purified from hybridoma cell culture

Synonym(s):

ARF6 Antibody - Anti-ARF6 antibody, Mouse monoclonal, Arf6 Antibody, Anti-ADP-Ribosylation Factor 6

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$650.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD08705572

Conjugate:

unconjugated

Clone:

ARFAG, monoclonal

Application:

Citations:

$650.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ARFAG, monoclonal

form

buffered aqueous solution

mol wt

antigen 18 kDa

species reactivity

bovine, canine, rat, human

concentration

2 mg/mL

technique(s)

western blot: 1-2 μg/mL using MDCK total cell extract.

isotype

IgG1

UniProt accession no.

P62330

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ARF6(382)
rat ... Arf6(79121)

Description

General description

ARF6 is a member of the ADP-ribosylation factor (ARF) family. It is specifically involved in endocytosis of plasma membrane proteins.

Anti-ARF6 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ARFAG produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. class III includes only human Arf6. The ADP-ribosylation factors (Arf) family of proteins, which belong to the Ras (rat sarcoma viral oncogene homolog) family, are low molecular weight proteins capable of binding GTP.

Application

Anti-ARF6 antibody, Mouse monoclonal has been used in:

western blotting
enzyme linked immunosorbent assay (ELISA)
immunocytochemistry

Mouse Monoclonal Anti-ARF6 antibody can be used for western blot assays at 1-2μg/ml using MDCK cell extracts. The antibody can also be used for ELISA and immunocytochemistry.

Biochem/physiol Actions

ADP-ribosylation factor 6 (Arf6) is present at the plasma membrane and on endosomal membranes where it regulates the flow of trafficking into and out of the cell, and regulates the actin cytoskeleton on the plasma membrane. ARF6 activates phospholipase D (PLD) and phosphatidylinositol 4-phosphate 5-kinase (PIP5K) to generate phosphatidic acid and PIP2 (phosphotidylinositol 4,5-bisphosphate), respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8604	AV54598	MABS2041
Sigma-Aldrich A5230 Anti-ARF6 antibody, Mouse monoclonal	Sigma-Aldrich A8604 Anti-Annexin V antibody, Mouse monoclonal	Sigma-Aldrich AV54598 Anti-ARF6 antibody produced in rabbit	Sigma-Aldrich MABS2041 Anti-pan-ARF Antibody, clone 1D9
description clone ARFAG, purified from hybridoma cell culture	description clone AN5, purified from hybridoma cell culture	description affinity isolated antibody	description clone 1D9, from mouse
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin
Gene Information human ... ARF6(382) rat ... Arf6(79121)	Gene Information human ... ANXA5(308)	Gene Information human ... ARF6(382)	Gene Information human ... ARF1(375)
clone ARFAG, monoclonal	clone AN5, monoclonal	clone polyclonal	clone 1D9, monoclonal
biological source mouse	biological source mouse	biological source rabbit	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity bovine, canine, rat, human	species reactivity human	species reactivity mouse, rabbit, bovine, human, dog, sheep, horse, rat, guinea pig	species reactivity human

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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