In order to achieve 2% BSA, dissolve 100 mL of PBS in 2 G of BSA. So, dissolving 20 G of BSA in 1000 mL of PBS would give 2% BSA.
A5611
牛血清白蛋白 来源于牛血清
Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis)
同義詞:
BSA, 牛白蛋白, 牛血清白蛋白
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CAS 編號:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.25
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥96% (agarose gel electrophoresis)
Form:
lyophilized powder
Technique(s):
immunohistochemistry: suitable
暫時無法取得訂價和供貨情況
biological source
bovine
Quality Segment
assay
≥96% (agarose gel electrophoresis)
form
lyophilized powder
packaging
poly bottle of
origin
USA origin
technique(s)
immunohistochemistry: suitable
pH
4.8-7.5
solubility
H2O: soluble 40 mg/mL, clear to slightly hazy, yellow
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Serum albumins are soluble constituents of the circulatory system. Bovine serum albumin (BSA) is homologous to human serum albumin (HSA). BSA consists of three homologous domains (I, II, III), that are separated into nine loops by disulfide bonds. Serum albumin acts as a depot protein.
Application
Bovine Serum Albumin has been used:
- for in vitro high molecular weight (HMW)-species formation assay
- for immunohistochemistry
- in Escherichia coli quantitative polymerase chain reaction (qPCR) analysis
Biochem/physiol Actions
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Preparation Note
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
This is a cold alcohol fractionated albumin that is produced by Sigma-Aldrich with a stricter adherence to the original Cohn method of fractionation than other commercially available bovine albumins.
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本產品 | |||
|---|---|---|---|
| description Cohn Fraction V, lyophilized powder, ≥96% (agarose gel electrophoresis) | description lyophilized powder, ≥96% (agarose gel electrophoresis) | description heat shock fraction, lyophilized powder, essentially fatty acid free, ≥98% (agarose gel electrophoresis) | description lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis) |
| assay ≥96% (agarose gel electrophoresis) | assay ≥96% (agarose gel electrophoresis) | assay ≥98% (agarose gel electrophoresis) | assay ≥96% (agarose gel electrophoresis) |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) immunohistochemistry: suitable | technique(s) ELISA: suitable, blood typing: suitable, microbiological culture: suitable | technique(s) cell culture | mammalian: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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存儲類別/等級
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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How can I prepare 1L of PBS with 2% of BSA
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