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A6007

Apotryptophanase from Escherichia coli

soluble powder, 75-150 units/mg solid

Synonym(s):

Tryptophanase from Escherichia coli, L-Tryptophan indole-lyase (deaminating)

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25 mg
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$752.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
75-150 units/mg solid
Biological source:
Escherichia coli

$752.00


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biological source

Escherichia coli

Quality Level

form

soluble powder

specific activity

75-150 units/mg solid

storage temp.

−20°C

Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency [1].

Biochem/physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase [1].

Other Notes

One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.

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This Item
T7677G8880G5921
specific activity

75-150 units/mg solid

specific activity

≥0.1 unit/mg solid

specific activity

≥50 units/mg protein

specific activity

≥5.0 units/mg solid

biological source

Escherichia coli

biological source

-

biological source

Escherichia coli

biological source

-

form

soluble powder

form

powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Christian Starkenmann et al.
Journal of agricultural and food chemistry, 59(7), 3358-3365 (2011-03-08)
The objective of this study was to verify whether the volatile organic sulfur compounds recently discovered in bell pepper (Capsicum annuum, L. cultivars), such as the mercapto-ketones: 4-sulfanyl-2-heptanone and 2-sulfanyl-4-heptanone, the mercapto-alcohols: 4-sulfanyl-2-heptanol and 2-sulfanyl-4-heptanol, and heptane-2,4-dithiol, originate from their
Dorota Kuczyńska-Wiśnik et al.
Research in microbiology, 161(10), 847-853 (2010-09-28)
Recent studies have revealed that antibiotics can promote the formation of reactive oxygen species which contribute to cell death. In this study, we report that five different antibiotics known to stimulate production of reactive oxygen species inhibited growth of Escherichia
Leonardo G Trabuco et al.
Structure (London, England : 1993), 18(5), 627-637 (2010-05-14)
Regulatory nascent chains interact with the ribosomal exit tunnel and modulate their own translation. To characterize nascent chain recognition by the ribosome at the atomic level, extensive molecular dynamics simulations of TnaC, the leader peptide of the tryptophanase operon, inside



Global Trade Item Number

SKUGTIN
A6007-25MG04061833376690

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