This product has not been specifically tested for suitability in SCSA and sperm DNA structure assays. However, there are a number of publications that have used this product for such applications. A thorough review of the literature is recommended. Please see the link below to review one such publication:
https://www.sciencedirect.com/science/article/pii/S0188440901003289
A6014
Acridine Orange hemi(zinc chloride) salt
For nucleic acid staining in cells or gels
Sinonimo/i:
3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14
per visualizzare i prezzi organizzativi e contrattuali.
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
10 g | Per conoscere la disponibilità, visualizza il carrello | CHF 99.70 |
25 g | Per conoscere la disponibilità, visualizza il carrello | CHF 232.00 |
100 g | Per conoscere la disponibilità, visualizza il carrello | CHF 1800.00 CHF 1530.00 |
Informazioni su questo articolo
Formula condensata:
C17H20ClN3 · HCl · 1/2ZnCl2
Numero CAS:
Peso molecolare:
369.96
EC Number:
233-353-6
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Colour Index Number:
46005
Beilstein/REAXYS Number:
3734978
CHF 99.70
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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Permettici di aiutartiQuality Segment
product line
BioReagent
form
powder
composition
Dye content, ~80%
technique(s)
nucleic acid detection: suitable
solubility
ethanol: 2 mg/mL, 4 mg/mL (2-methoxyethanol (EGME)), water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
suitability
suitable for flow cytometry, suitable for microscopy
storage temp.
room temp
SMILES string
Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
InChI
1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
InChI key
RAHGLSRJKRXOSY-UHFFFAOYSA-L
General description
Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.
Application
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Suitable for
- detection of nucleic acids separated by gel electrophoresis
- fluorescence and epifluorescence microscopy
- analysis of mitochondria and lysosomes by flow cytometry
- DNA staining in apoptosis studies
Biochem/physiol Actions
Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.[1]
Features and Benefits
- 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
- differential staining of single- and double-stranded polynucleotides
1 of 1
Questo articolo | |||
|---|---|---|---|
| technique(s) nucleic acid detection: suitable | technique(s) - | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| suitability suitable for flow cytometry, suitable for microscopy | suitability - | suitability - | suitability - |
| solubility ethanol: 2 mg/mL, water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.), 4 mg/mL (2-methoxyethanol (EGME)) | solubility H2O: 1 mg/mL, clear, orange to very deep orange | solubility 1 M HCl: 1 mg/mL, clear | solubility H2O: 0.1%, clear, orange to red |
| form powder | form powder | form powder | form powder |
| composition Dye content, ~80% | composition Dye content, 85% | composition Dye content, 75% | composition - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is product A6014, Acridine Orange hemi(zinc chloride) salt, used for sperm chromatin structure assay (SCSA) and sperm DNA structure assay?
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