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A6691

Amidase from Pseudomonas aeruginosa

recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)

Synonym(s):

Acrylamide Amidohydrolase, Acylase

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250 units
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€393.00
1000 units
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€752.00

About This Item

CAS Number:
9012-56-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.5.1.4 (BRENDA, IUBMB)
MDL number:
MFCD00166319
Recombinant:
expressed in E. coli
Concentration:
14 mg/mL

€393.00

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recombinant

expressed in E. coli

Quality Segment

200

form

buffered aqueous glycerol solution

hydroxamate transferase activity

≥200 units/mg protein (biuret)

concentration

14 mg/mL

UniProt accession no.

Q9HWL8

storage temp.

−20°C

Gene Information

Pseudomonas aeruginosa PAO1 ... PA4163(880181)

Application

The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .
Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.

Biochem/physiol Actions

The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .

Physical form

Solution in 50% glycerol containing 7 mM 2-mercaptoethanol and phosphate buffer salt

Other Notes

One unit will convert 1.0 μmole of acetamide and hydroxylamine to acetohydroxamate and ammonia per min at pH 7.2 at 37 °C.

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Amidase from Pseudomonas aeruginosa recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)

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Sigma-Aldrich

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description

recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)

description

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

description

Grade V, lyophilized powder, ≥50 units/mg protein

description

5-10 units/mg protein

Gene Information

Pseudomonas aeruginosa PAO1 ... PA4163(880181)

Gene Information

cow ... ADA(280712)

Gene Information

-

Gene Information

-

concentration

14 mg/mL

concentration

-

concentration

-

concentration

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

suspension

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

UniProt accession no.

Q9HWL8

UniProt accession no.

P56658

UniProt accession no.

-

UniProt accession no.

-

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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