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Anti-AtMPK4 antibody produced in rabbit

Name: Anti-AtMPK4 antibody produced in rabbit
Brand: SIGMA
Price: 799 USD
Availability: InStock

affinity isolated antibody, liquid

Synonym(s):

Anti-Arabidopsis thaliana MPK4

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Size/SKU	Availability	Price
200 μL	Check Cart for Availability	$799.00 $799.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.44

MDL number:

MFCD07370260

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

$799.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

grade

Molecular Biology

clone

polyclonal

form

liquid

mol wt

antigen 43 kDa

species reactivity

Arabidopsis thaliana

technique(s)

western blot: 0.5-1 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana and Nicotiana tabacum leaves

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Arabidopsis thaliana ... ATMPK4(828151)

Description

General description

Anti-AtMPK4 is produced in rabbit using as immunogen a synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (Anti- mitogen-activated protein kinase-4), conjugated to KLH. In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant mitogen-activated protein kinase (MAPKs) are classified into the plant extracellular-regulated kinase (PERK) subfamily.

Mitogen-activated protein kinase-4 (MPK4) is expressed in the nucleus. In pre-mitotic and interphase cells, it is found in the cytoplasm.

Immunogen

synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (amino acids 365-376). This sequence is specific to AtMPK4 (not found in the other 20 known AtMPK isoforms). It shares limited homology (50-60% identity, continuous epitope) to the AtMPK5 and AtMPK12 isoforms.

Application

Anti- mitogen-activated protein kinase-4 (AtMPK4) antibody produced in rabbit has been used for Western blotting.

Anti-AtMPK4 antibody has been used in

immunoblotting
immunoprecipitation
co-immunoprecipitation
western blotting

Biochem/physiol Actions

In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4, and AtMPK6, and all three mitogen-activated protein kinase (MAPKs) have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold, and touch. Arabidopsis MAPKs, Anti- mitogen-activated protein kinase-3 (AtMPK3), Anti- mitogen-activated protein kinase-4 (AtMPK4), and Anti- mitogen-activated protein kinase-6 (AtMPK6) are rapidly and transiently activated by stress responses, by activation of distinct signal transduction pathways, involving upstream MAPKKKs (e.g. AtANP1 and AtMEKK1) and MAPKKs including AtMKK1/2 and AtMKK4/5.

Mitogen-activated protein kinase-4 (MPK4) negatively modulates systemic-acquired resistance. It functions as a regulator of retrograde signaling for photosynthesis, growth and immune defenses. It also has a role in cytokinesis and microtubule organization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

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This Item	M8318	A7104	M9818
Sigma-Aldrich A6979 Anti-AtMPK4 antibody produced in rabbit	Sigma-Aldrich M8318 Anti-AtMPK3 antibody produced in rabbit	Sigma-Aldrich A7104 Anti-AtMPK6 antibody produced in rabbit	Sigma-Aldrich M9818 Anti-Myopodin antibody produced in rabbit
description affinity isolated antibody, liquid	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, liquid	description affinity isolated antibody, buffered aqueous solution
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) western blot: 0.5-1 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana and Nicotiana tabacum leaves	technique(s) western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves, western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves	technique(s) western blot: 0.25-0.5 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves	technique(s) immunohistochemistry (frozen sections): 1-2 μg/mL using rat skeletal muscle and rat kidney, western blot: 1-2 μg/mL using cytosolic fraction of rat skeletal muscle
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c