This product is horseradish peroxidase conjugated.
A7058
Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal
clone HIS-1, purified from hybridoma cell culture
Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal
Synonym(s):
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 vial | Please contact Customer Service for Availability | $1,360.00 |
About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
HIS-1, monoclonal
Application:
WB
Citations:
152
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
storage temp.
2-8°C
target post-translational modification
unmodified
General description
The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Immunogen
recombinant polyHistidine tagged fusion protein.
Application
Antibody suitable for immunoblotting. Working dilution 1:20
Also suitable for dot blot assays and ELISA
Also suitable for dot blot assays and ELISA
Biochem/physiol Actions
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
The antibody conjugate should be reconstituted with 0.5 ml deionized water.
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
1 of 1
This Item | |||
|---|---|---|---|
| clone HIS-1, monoclonal | clone HIS-1, monoclonal | clone HIS-1, monoclonal | clone 4A7, monoclonal |
| antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form ascites fluid | antibody form purified from hybridoma cell culture |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate peroxidase conjugate | conjugate - | conjugate unconjugated | conjugate unconjugated |
| technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) flow cytometry: 3-6 μg/test, immunoblotting: 0.25-0.5 μg/mL, immunofluorescence: 4-8 μg/mL | technique(s) dot blot: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells., western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells. |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7058-1VL | 04061838443632 |
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To whom it may concern. Is the antibody Anti-his 6 tag hrseradish Peroxidase conjugated? Thank you
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