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Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit

Name: Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Glutathione-S-Transferase, Anti-GST

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About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d), WB

Citations:

Pricing and availability is not currently available.

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Properties

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 27.5 kDa

technique(s)

direct ELISA: 1:10,000, western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in

immunoblotting
affinity pull-down assay
western blotting
enzyme linked immunosorbent assay (ELISA)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs.

Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G7781	G1160	SAB4200237
$Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A7340 Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit	$Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G7781 Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit	Sigma-Aldrich G1160 Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse	Sigma-Aldrich SAB4200237 Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal
description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description clone GST-2, ascites fluid	description clone 2H3-D10, purified from hybridoma cell culture
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form ascites fluid	antibody form purified immunoglobulin
conjugate peroxidase conjugate	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
mol wt antigen 27.5 kDa	mol wt -	mol wt -	mol wt -
technique(s) direct ELISA: 1:10,000, western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST	technique(s) dot blot: suitable, indirect ELISA: 1:10,000, western blot: 1:2,000 using lysates of induced Escherichia coli expressing GST	technique(s) dot blot: suitable, indirect ELISA: suitable, western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST	technique(s) western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c