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A7340

Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Glutathione-S-Transferase, Anti-GST

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0.5 mL
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$1,120.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB
Citations:
35

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biological source

rabbit

Quality Segment

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 27.5 kDa

technique(s)

direct ELISA: 1:10,000, western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Glutathione-S-Transferase (GST) belongs to a family of Phase II detoxification enzymes which are involved in catalyzing the conjugation of glutathione (GSH) with many exogenous and endogenous electrophilic substances. GST is of two kinds- membrane-bound microsomal and cytosolic. The cytosolic GSTs are made of six classes: α, μ, ω, π, θ, and ζ.
The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Anti-Glutathione-S-Transferase (GST)-Peroxidase Conjugate antibody has been used in
  • immunoblotting
  • affinity pull-down assay
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Glutathione-S-Transferase (GST) are involved in protecting cellular macromolecules from the reactive electrophiles. The μ and π transferases regulate the mitogen-activated protein (MAP) kinase pathway. GSTs help in building up cellular resistance against microbial antibiotic, herbicides, insecticides, chemotherapy agents and drugs.
Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A7340

Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

G7781

Anti-Glutathione-S-Transferase (GST) antibody produced in rabbit

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal clone GST-2, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200692

Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal

Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse clone GST-2, ascites fluid

Sigma-Aldrich

G1160

Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse

description

IgG fraction of antiserum, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

clone GST-2, purified from hybridoma cell culture

description

clone GST-2, ascites fluid

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

purified immunoglobulin

antibody form

ascites fluid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

mouse

biological source

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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