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A7437

Acetate Kinase from Escherichia coli

lyophilized powder, ≥150 units/mg protein (biuret)

Synonyme(s) :

ATP:Acetate phosphotransferase

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250 units
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164,00 €
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A propos de cet article

Numéro CAS:
9027-42-3
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Numéro CE :
2.7.2.1 (BRENDA, IUBMB)
MDL number:
MFCD00130330
Specific activity:
≥150 units/mg protein (biuret)

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form

lyophilized powder

Quality Segment

200

specific activity

≥150 units/mg protein (biuret)

composition

Protein, 5.0-20.0%

UniProt accession no.

P0A6A3

foreign activity

adenosine triphosphatase and NADH oxidase ≤0.01%

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... ackA(946775)

General description

Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily.[1] It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.

Application

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation [2].
Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.

Biochem/physiol Actions

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA [3]. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.
Involved in the metabolism of propanoate, pyruvate and taurine

Physical form

Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

Other Notes

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

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Cet article
G4509G1270G6278
Acetate Kinase from Escherichia coli lyophilized powder, ≥150 units/mg protein (biuret)

Sigma-Aldrich

A7437

Acetate Kinase from Escherichia coli

Glycerokinase from Escherichia coli lyophilized powder, 40-100 units/mg protein

Sigma-Aldrich

G4509

Glycerokinase from Escherichia coli

L-Glutamine Synthetase from Escherichia coli lyophilized powder, 400-2,000 units/mg protein

Sigma-Aldrich

G1270

L-Glutamine Synthetase from Escherichia coli

Glycerokinase from Escherichia coli 300-600 units/mL, ammonium sulfate suspension

Sigma-Aldrich

G6278

Glycerokinase from Escherichia coli

description

lyophilized powder, ≥150 units/mg protein (biuret)

description

lyophilized powder, 40-100 units/mg protein

description

lyophilized powder, 400-2,000 units/mg protein

description

300-600 units/mL, ammonium sulfate suspension

specific activity

≥150 units/mg protein (biuret)

specific activity

40-100 units/mg protein

specific activity

400-2,000 units/mg protein

specific activity

-

Gene Information

Escherichia coli K12 ... ackA(946775)

Gene Information

-

Gene Information

Escherichia coli K12 ... glnA(948370)

Gene Information

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

UniProt accession no.

P0A6A3

UniProt accession no.

-

UniProt accession no.

P0A9C5

UniProt accession no.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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