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A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

Synonym(s):

ATP phosphohydrolase, ATPase

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5 units
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$252.00
5 x 5 units
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$886.00

About This Item

CAS Number:
9000-83-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.6.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00135671
Specific activity:
≥0.3 units/mg protein, pH 7.8
Biological source:
Porcine cerebral cortex

$252.00

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biological source

Porcine cerebral cortex

Quality Segment

200

description

Sodium and Potassium-Activated

form

lyophilized powder

specific activity

≥0.3 units/mg protein, pH 7.8

composition

Protein, 5.0-25.0% biuret

technique(s)

inhibition assay: suitable

UniProt accession no.

P05024, P05027

storage temp.

−20°C

Gene Information

pig ... ATP1A1(397481), ATP1B1(396898)

Application

ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity [1].

Biochem/physiol Actions

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells [1].

Physical form

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl

Preparation Note

Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+.

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This Item
P6887SAB5700279A276
Adenosine 5′-Triphosphatase from porcine cerebral cortex lyophilized powder, ≥0.3 units/mg protein, pH 7.8

Sigma-Aldrich

A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

Pepsin from porcine gastric mucosa lyophilized powder, ≥3,200 units/mg protein

Sigma-Aldrich

P6887

Pepsin from porcine gastric mucosa

Anti-Phospho-ATM-S1981 antibody produced in rabbit

Sigma-Aldrich

SAB5700279

Anti-Phospho-ATM-S1981 antibody produced in rabbit

Monoclonal Anti-Na+/K+ ATPase (α Subunit) antibody produced in mouse clone M7-PB-E9, ascites fluid

Sigma-Aldrich

A276

Monoclonal Anti-Na+/K+ ATPase (α Subunit) antibody produced in mouse

specific activity

≥0.3 units/mg protein, pH 7.8

specific activity

≥3,200 units/mg protein

specific activity

-

specific activity

-

Gene Information

pig ... ATP1A1(397481), ATP1B1(396898)

Gene Information

pig ... LOC396892(396892)

Gene Information

human ... ATM(472)

Gene Information

human ... ATP1A1(476)

biological source

Porcine cerebral cortex

biological source

Porcine gastric mucosa

biological source

rabbit

biological source

mouse

technique(s)

inhibition assay: suitable

technique(s)

-

technique(s)

immunofluorescence: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

immunocytochemistry: suitable, immunofluorescence: 1:20, immunohistochemistry (frozen sections): 1:100, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:500-1:5000

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

-

UniProt accession no.

P05024, P05027

UniProt accession no.

P00791

UniProt accession no.

Q13315

UniProt accession no.

P05023

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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